不同炮制去心法对巴戟天耐斯糖含量的影响

目的：比较不同炮制去心法对巴戟天耐斯糖含量的影响。方法采用HPLC-ELSD 法，色谱柱为 Welch AQ-C18（250 mm×4.6 mm，5μm）；流动相为甲醇-水（3∶97）；柱温为25℃；流速为0.8 mL/min；进样量为10μL；漂移管温度为40℃；低温雾化。结果采用不同炮制去心法处理后，巴戟天中耐斯糖的含量高低为：润法＞煮法＞清蒸法＞盐蒸法＞泡后蒸法＞泡法。结论采用润法去心，巴戟天中耐斯糖损失最少。

Influence of Different Methods for Removing Woody Core on Nystose Content in Morinda officinalis

Objective To compare the influence of different methods for removing woody core on nystose content in Morinda officinalis. Methods The HPLC-ELSD detection was used with the chromatographic column Welch AQ-C18 (250 mm× 4.6 mm, 5 μm),the mobile phase was methanol-water (3∶97),the column temperature was at 25 ℃,the flow rate was 0.8 mL/min,the injection volume was 10 μL, and the drift tube temperature was at 40 ℃ with low temperature atomization. Results The level of the nystose content in Morinda officinalis with different process was: moistening method>cooking method>steaming method>steaming method with salt>steaming method after soaking>soaking method. Conclusion The nystose content in Morinda officinalis is the least loss when use the moistening method to removing woody core.