复方紫草油促进实验兔创面碱性成纤维细胞生长因子及其基因的表达和组织学变化

目的:通过观察不同创面的组织学变化、碱性成纤维细胞生长因子(basic fibroblast growth factor,bFGF)mRNA的表达和创面的愈合率来探讨紫草油促进创面愈合的分子生物学机制。方法:采用新西兰白兔制作背部皮肤缺损模型,共18只,将每侧的创面随机分为2组,复方紫草油组用紫草油对创面进行治疗,对照组用凡士林纱布治疗。用组织学、组织化学、电镜等方法观察创面的组织结构和愈合率、用Western-blot,RT-PCR方法观察2组的bFGF及其mRNA表达。结果:复方紫草油组和对照组的愈合率有显著性差异(P<0.05),复方紫草油组愈合率高。复方紫草油组创面的成纤维细胞,胶原和毛细血管比对照组多。bFGF及其基因表达在各个时段均明显超过对照组。创面组织中bFGF/-βactin灰密度值有显著性差异(P<0.05);创面愈合率与创面组织中bFGF及其基因表达程度相平行,在创面愈合的不同阶段中,作为内参的-βactin基因都有表达且无显著变化(P>0.05)。结论:在紫草油对创面愈合过程中bFGF有明显的促进作用,bFGF对创伤愈合具有重要的调节作用,紫草油可能是通过促进细胞分泌bFGF的增长来完成创面的修复过程。

Arnebia root oil promotes histological change and up-regulates bFGF and it's mRNA expression in the raw surface of the rabbits

Objective: To explore the molecular biological mechanism of arnebia root oil in promoting wound surface healing by observing histological change and basic fibroblast growth factor(bFGF) mRNA expression in the wound surface tissues of 2 groups,as well as the wound surface healing rate.Method: Experimental model of incised-wound was produced on the back of 18 New Zealand albino rabbits.The wound surfaces were randomly divided into two groups,namely,experimental group and control group.The wound surfaces in the experimental group were treated by arnebia root oil and those in control group were treated by petrolatum gauze.Then raw surfaces were evaluated by the techniques of histology,histochemistry and electron microscope and the healing rates of the raw surfaces were compared between the two groups.Content of bFGF and it's mRNA expression in wound surface tissue was also measured by means of Western-blot and RT-PCR.Result: The wound surface healing rate in experimental group was higher than that in control group(P<0.05).The fibroblast,collagen and blood capillaries were comparatively richer in experimental group as compared with those in control group,and similarly,the expression of bFGF mRNA was also significantly enhanced in the experimental group as compared with control group during the various periods of treatment.In addition,the changes in the expressions of bFGF and it's mRNA paralleled the changes of healing rates in the two groups.Conclusion: the present results showed that arnebia root oil significantly can promote the healing of raw surfaces,which may be mediated by up-regulation of bFGF expression.