| 狼疮鼠淋巴细胞IgG2aVH区反义RNA重组体的构建及鉴定 |
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| 摘 要： |
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| 关 键 词： | 淋巴细胞 cDNA克隆 反义RNA 小鼠 系统性红斑狼疮 |
Construction of recombinant antisense RNA IgG2aVH region of murine systemic lupus erythematous] |
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| Authors: | J W Deng Q J Lu T T Hu |
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| Institution: | Center Research Laboratory, Second Xiangya Hospital, Central South University, Changsha, 410011, China. |
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| Abstract: | OBJECTIVE: To construct recombinant plasmid of IgG2aVH region antisense RNA of systemic lupus erythematous. METHODS: Total RNA was isolated from spleen cell of BWF1 mice. Using spleen cell total RNA as the template, We designed specific primers from A6.1 region sequences, and amplified the IgG2aVH region 375 bp DNA fragments by RT-PCR. The IgG2aVH cDNA was cloned by T/A and inserted into pcDNA 3.1 plasmid of vector. RESULTS: The IgG2aVH antisense RNA plasmid expressing recombinant was identified by restriction enzyme digestion and DNA sequence analysis. CONCLUSION: There is IgG2aVH gene in F1 mice spleen cell; the IgG2aVH antisense RNA expressing recombinants is constructed successfully. |
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