Peroxidative in vitro metabolism of diethylstilbestrol induces formation of 8-hydroxy-2'-deoxyguanosine

The generation of superoxide and hydroxyl radicals is knownto be implicated in the hydroxylation of 2'-deoxyguanosine (dG)at the C-8 position and of guanine base residues in DNA. Itwas also shown previously that in the presence of horseradishperoxidase, hydrogen peroxide and Fe³⁺-EDTA complex, diethylstilbestrol(DES) induces single strand breaks in DNA, caused by the productionof superoxide anion (O^–) and hydroxyl (OH) radicals. Bymeans of high-pressure liquid chromatography and electrochemicaldetection a strong indication is adduced that dG is oxidizedto 8-hydroxy- 2'-deoxyguanosine during peroxidative in vitrometabolism of DES, which might be at the basis of DES inducedcell transformation.