白藜芦醇对脂多糖诱导的小鼠重症肺炎的保护作用

目的:探讨白藜芦醇对实验性小鼠重症肺炎的保护作用及机制.方法:建立脂多糖吸入所致的小鼠实验性急性重症肺炎模型,然后给予白藜芦醇腹腔注射给药,观察小鼠肺组织病理、肺组织湿/干质量比、支气管肺泡灌洗液中细胞分类计数变化,并检测肺组织中TNF-α、IL-1β、IL-6等炎症因子的表达差异.结果:实验性急性重症肺炎模型小鼠在接受白藜芦醇3～4 d后症状开始减轻,肺部炎症缓解.白藜芦醇治疗后,小鼠肺泡灌洗液中单核巨噬细胞、淋巴细胞、中性粒细胞及白细胞中性粒细胞计数和百分比均小于模型组,肺组织中TNF-α、IL-1β、IL-6的表达均显著低于模型组.结论:白藜芦醇有一定的抗炎作用,可以缓解小鼠重症肺炎症状,其机制可能是降低炎性肺组织中TNF-α、IL-1β、IL-6等的含量,从而减轻炎症反应.

Protective effect of Resveratrol on LPS-inducing mice acute lung inflammation

To study the protective effect of Resveratrol on the acute lung inflammation in experimental mice and its mechanism of action. METHODS： The acute lung inflammation model was established in mice and mice were i.p. treated with Resveratro or vehicle in 4 d. The curative effect of Resveratrol were observed, as well as the changes of differential leukocytes count in bronchoalveolar lavage fluid, the expression of TNF-a IL-β and IL-6 after treatment. RESULTS： The results showed that Resveratrol could significantly relieve the clinical symptoms of acute lung inflammation and couldreduce the numbers and percentages of leukocytes in the bronehoalveolar lavage fluid. The activation of TNF-a IL-βand IL-6 were inhibi-ted by Resveratrol. CONCLUSION. These findings indicated that Resveratrol possessed reliable curative effect for acute lung inflammation, in which the inhibition of inflammatory factors, especially TNF-α, 1L-β and IL-6 was a potential antiphlogistic mechanism.