| Steatosis recovery after treatment with a balanced sunflower or olive oil-based diet： Involvement of perisinusoidal stellate cells |
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| 引用本文： | Hernández R,Martínez-Lara E,Cañuelo A,del Moral ML,Blanco S,Siles E,Jiménez A,Pedrosa JA,Peinado MA. Steatosis recovery after treatment with a balanced sunflower or olive oil-based diet： Involvement of perisinusoidal stellate cells[J]. World journal of gastroenterology : WJG, 2005, 11(47): 7480-7485. DOI: 10.3748/wjg.v11.i47.7480 |
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| 作者姓名： | Hernández R Martínez-Lara E Cañuelo A del Moral ML Blanco S Siles E Jiménez A Pedrosa JA Peinado MA |
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| 作者单位： | Department of Experimental Biology University of Jaén,Paraje Las Lagunillas s/n,E-23071 Jaén,Spain,Department of Experimental Biology,University of Jaén,Paraje Las Lagunillas s/n,E-23071 Jaén,Spain,Department of Experimental Biology,University of Jaén,Paraje Las Lagunillas s/n,E-23071 Jaén,Spain,Department of Experimental Biology,University of Jaén,Paraje Las Lagunillas s/n,E-23071 Jaén,Spain,Department of Experimental Biology,University of Jaén,Paraje Las Lagunillas s/n,E-23071 Jaén,Spain,Department of Experimental Biology,University of Jaén,Paraje Las Lagunillas s/n,E-23071 Jaén,Spain,Department of Experimental Biology,University of Jaén,Paraje Las Lagunillas s/n,E-23071 Jaén,Spain,Department of Experimental Biology,University of Jaén,Paraje Las Lagunillas s/n,E-23071 Jaén,Spain,Department of Experimental Biology,University of Jaén,Paraje Las Lagunillas s/n,E-23071 Jaén,Spain |
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| 基金项目： | Supported by Direction General de Investigation Cientifica y Tecnica, SAF 2003 04398-C02-02, and Junta de Andalucia,CVI-0184 |
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| 摘 要： | AIM: To analyze the relationship between perisinusoidal stellate cell (PSC) activation and the dietary fat quantity and composition in the treatment of hepatic steatosis. METHODS: Using an experimental rat model of steatosis based on the intake of a hyperlipidic diet (14% fat as olive oil or sunflower oil, HL-O and HL-S, respectively), we analyzed the liver's capability of recovery after the treatment with a normal-lipidic diet (5% fat as olive oil or sunflower oil, NL-O and NL-S, respectively) by immu-nocytochemical and Western blot analysis of glial fibril-lary acidic protein (GFAP) expression in PSCs, collagen quantification and serum aminotransferase determination. RESULTS: The fatty infiltration in the steatotic livers decreased after the treatment with both NL diets, indicating liver recovery. This decrease was accompanied with a lower collagen deposition and aminotransferase level as well as changes in the PSC population that increased the GFAP expression. The above-mentioned effects were more pronounced in animals fed on NL-O based diet. CONCLUSION: Treatment with a balanced diet enriched in olive oil contributes to the liver recovery from a steatotic process. The PSC phenotype is a marker of this hepatic-recovery model.
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| 关 键 词： | 脂肪变性 橄榄油 星形细胞 治疗 |
| 收稿时间： | 2005-01-18 |
Steatosis recovery after treatment with a balanced sunflower or olive oil-based diet: involvement of perisinusoidal stellate cells |
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| Hernández Raquel,Martínez-Lara Esther,Cañuelo Ana,del Moral Maria Luisa,Blanco Santos,Siles Eva,Jiménez Ana,Pedrosa Juan Angel,Peinado Maria Angeles. Steatosis recovery after treatment with a balanced sunflower or olive oil-based diet: involvement of perisinusoidal stellate cells[J]. World journal of gastroenterology : WJG, 2005, 11(47): 7480-7485. DOI: 10.3748/wjg.v11.i47.7480 |
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| Authors: | Hernández Raquel Martínez-Lara Esther Cañuelo Ana del Moral Maria Luisa Blanco Santos Siles Eva Jiménez Ana Pedrosa Juan Angel Peinado Maria Angeles |
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| Affiliation: | Department of Experimental Biology, University of Jaén, Paraje Las Lagunillas s/n, E-23071 Jaén, Spain |
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| Abstract: | AIM: To analyze the relationship between perisinusoidal stellate cell (PSC) activation and the dietary fat quantity and composition in the treatment of hepatic steatosis. METHODS: Using an experimental rat model of steatosis based on the intake of a hyperlipidic diet (14% fat as olive oil or sunflower oil, HL-O and HL-S, respectively), we analyzed the liver's capability of recovery after the treatment with a normal-lipidic diet (5% fat as olive oil or sunflower oil, NL-O and NL-S, respectively) by immu-nocytochemical and Western blot analysis of glial fibril-lary acidic protein (GFAP) expression in PSCs, collagen quantification and serum aminotransferase determination. RESULTS: The fatty infiltration in the steatotic livers decreased after the treatment with both NL diets, indicating liver recovery. This decrease was accompanied with a lower collagen deposition and aminotransferase level as well as changes in the PSC population that increased the GFAP expression. The above-mentioned effects were more pronounced in animals fed on NL-O based diet. CONCLUSION: Treatment with a balanced diet enriched in olive oil contributes to the liver recovery from a steatotic process. The PSC phenotype is a marker of this hepatic-recovery model. |
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| Keywords: | PSCs GFAP Steatosis Collagen Hyperlipidic diet Liver recovery Olive oil |
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