结肠癌组织P57kip2和Ki67表达对侵袭性的影响

目的:研究结肠癌中P57kip2与Ki67的表达与肿瘤的发生和发展的关系．方法:采用免疫组化技术ABC法检测P57kip2与Ki67抗原在正常结肠粘膜10例、结肠良性肿瘤10例和结肠癌69例组织中的表达．结果:正常结肠黏膜组织和结肠良性肿瘤组织中P57kip2和Ki67的表达没有统计学差异．结肠癌组织中P57kip2抗原表达的标志指数(labeling index,LI)较正常结肠黏膜组织和结肠良性肿瘤明显降低(P=0．0001);Ki67抗原表达LI较正常结肠黏膜组织和结肠良性肿瘤明显升高(P=0．0001)．不同性别、年龄结肠癌患者癌组织中P57kip2和Ki67的表达没有统计学差异．P57kip2在结肠癌低分化腺癌组织中的表达较在中高分化腺癌组织中的表达明显降低(P=0．032)．Ki-67在结肠癌低分化腺癌组织中的表达较在中高分化腺癌组织的表达明显升高(P=0．0226)．P57kip2在有淋巴管浸润的结肠癌组织中的表达较在无淋巴管浸润的结肠癌组织中的表达明显降低(P=0．0001)．Ki-67在有淋巴管浸润的结肠癌组织中的表达较在无淋巴管浸润的结肠癌组织中的表达明显升高(P=0．0001)．P57kip2在结肠癌Duke’s A期和B期的癌组织中的表达较在C期和D期癌组织中的表达明显升高(P=0．0002)．Ki-67在A期和B期的癌组织中的表达较在C期和D期癌组织中的表达明显降低(P=0．0006)．P57kip2在结肠癌T1,T2期的癌组织中的表达较在T3,T4期癌组织中的表达明显升高(P=0．0311)．Ki-67在T1, T2期的癌组织中的表达较在T3,T4期癌组织中的表达明显降低(P=0．0235)．P57kip2与Ki-67在结肠癌组织中的表达呈现明显的负相关(r= -0．847,P=0．0001)．结论:P57kip2表达下调,Ki67抗原表达上调在结肠癌的发生发展和转移过程中发挥着重要作用．

Expression of P57kip2 and Ki67 in colon adenocarcinoma

AIM: To investigate the expression of P57kip2 and Ki67 and their roles in the carcinogenesis and progression of colon adenocarcinoma. METHODS: Avidin-biotin-peroxidase complex (ABC) immunohistochemistry was used to detect the expression of P57kip2 and Ki67 in normal colon tissues (n = 10), colon benign tumor (n = 10), and colon adenocarcinoma (n = 69). RESULTS: The expression of P57kip2 and Ki67 were not significantly different between normal colon tissues and colon benign tumor (P > 0.05). The expression of P57kip2 in colon cancer was significantly lower than that in normal colon tissues and colon benign tumor (P = 0.0001), while the expression of Ki67 was markedly higher in colon cancer (P = 0.0001). The expression of P57kip2 and Ki67 were correlated with the age and sex of patients (P > 0.05). The expression of P57kip2 was significantly lower in the poorly-differentiated cancer than that in the well-differentiated cancer (P = 0.032), while the expression of Ki67 was just in the contrary condition (P = 0.0226). The expression of P57kip2 in the cancer tissues with lymphatic invasion was significantly lower than in those without lymphatic invasion (P = 0.0001), while the expression of Ki67 was also in the contrary condition (P = 0.0001). In the cancer tissues, the expression of P57kip2 in the Duke's stage A B and T1 T2 stage was significantly higher than that in the Duke's stage C D and T3 T4 stage (P = 0.0002; P = 0.0311), while the expression of Ki67 was in the contrary condition (P = 0.0006; P = 0.0235). The expression of P57kip2 were negatively correlated with the expression of Ki67 in the cancer tissues (r = -0.847, P = 0.0001). CONCLUSION: The decreased expression of P57kip2 and/or over-expression of Ki67 antigen may play important roles in the carcinogenesis, progression and metastasis of colon adenocarci-noma.