| Influence of High Level TGF-β1 on Scleral Thickness |
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| 引用本文： | 刘海霞,项楠,张虹.Influence of High Level TGF-β1 on Scleral Thickness[J].华中科技大学学报(医学英德文版),2007,27(5):601-604. |
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| 作者姓名： | 刘海霞 项楠 张虹 |
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| 作者单位： | Department of Ophthalmology Tongji Hospital Tongji Medical College Huazhong University of Science and Technology，Department of Ophthalmology Tongji Hospital Tongji Medical College Huazhong University of Science and Technology，Department of Ophthalmology Tongji Hospital Tongji Medical College Huazhong University of Science and Technology，Wuhan 430030 China，Wuhan 430030 China，Wuhan 430030 China |
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| 摘 要： | In order to explore the role of TGF-β1 in scleral remodeling and the possible mechanism,the influence of high level TGF-β1 on scleral thickness and the expression of MMP-2 and TIMP-2 was investigated in a TGF-β1 transgenic mouse model.Alb/TGF-β1(Cys223,225Ser) TGF-β1 transgenic mice were used as experimental subjects and non-transgenic littermates as controls.Plasma levels of TGF-β1 were determined by ELISA.TGF-β1,MMP-2 and TIMP-2 levels in sclera were detected by using Western blot.The thickness of posterior sclera was measured by computerized image analysis of a midsagittal section.Mean difference was analyzed with independent t-test.The results showed plasma levels of TGF-β1 in transgenic mice were 1.68 times as much as that in the controls(P<0.01).TGF-β1 levels in the sclera of transgenic mice were 2.68 times of the controls(P<0.01).Posterior scleral thickness in transgenic mice were significantly thicker than in the controls.There was no significant difference in the MMP-2 levels between transgenic mice and controls,but the TIMP-2 levels were increased significantly in transsgenic mice as compared with those in the controls.It was suggested that high levels of TGF-β1 in transgenic mice could result in the increased scleral thickness by inducing the expression of TIMP-2 to suppress the activity of MMP-2,finally inhibiting the degradation of collagen.
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| 关 键 词： | sclera TGF-β1 myopia |
| 收稿时间： | 2007-03-25 |
Influence of high level TGF-β1 on scleral thickness |
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| Liu?Haixia,Xiang?Nan,Zhang?Hong.Influence of high level TGF-β1 on scleral thickness[J].Journal of Zuazhong University of Science and Technology: Medical Edition,2007,27(5):601-604. |
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| Authors: | Liu Haixia Xiang Nan Zhang Hong |
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| Institution: | (1) Department of Ophthalmology, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, 430030, China |
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| Abstract: | Summary In order to explore the role of TGF-β1 in scleral remodeling and the possible mechanism, the influence of high level TGF-β1
on scleral thickness and the expression of MMP-2 and TIMP-2 was investigated in a TGF-β1 transgenic mouse model. Alb/TGF-β1
(Cys223,225Ser) TGF-β1 transgenic mice were used as experimental subjects and non-transgenic littermates as controls. Plasma levels of
TGF-β1 were determined by ELISA. TGF-β1, MMP-2 and TIMP-2 levels in sclera were detected by using Western blot. The thickness
of posterior sclera was measured by computerized image analysis of a midsagittal section. Mean difference was analyzed with
independent t-test. The results showed plasma levels of TGF-β1 in transgenic mice were 1.68 times as much as that in the controls (P<0.01). TGF-β1 levels in the sclera of transgenic mice were 2.68 times of the controls (P<0.01). Posterior scleral thickness in transgenic mice were significantly thicker than in the controls. There was no significant
difference in the MMP-2 levels between transgenic mice and controls, but the TIMP-2 levels were increased significantly in
transsgenic mice as compared with those in the controls. It was suggested that high levels of TGF-β1 in transgenic mice could
result in the increased scleral thickness by inducing the expression of TIMP-2 to suppress the activity of MMP-2, finally
inhibiting the degradation of collagen. |
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| Keywords: | sclera TGF-β 1 myopia |
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