纯二氧化碳气体对卵巢癌细胞生长及运动能力的影响

目的:观察纯CO2气体环境对卵巢癌细胞SKOV-3生长及运动能力的影响。方法:将体外培养的卵巢癌SKOV-3细胞悬液接种于96孔板,将培养板分别暴露于纯CO2环境1、2、3h,对照组放入常规细胞培养箱培养。纯CO2处理后24、48、72、96、120h,用四唑盐(MTT法)比色试验测定各组细胞的比色值,观察细胞生长情况,绘制细胞生长曲线,观察各组细胞倍增时间。采用划痕(W ound-assay)试验测定各组细胞随机运动能力的变化。结果:卵巢癌细胞SKOV-3暴露于纯CO2环境后第24、48、72小时MTT值与对照组无显著性差异,第96小时后其MTT值明显高于对照组,其差异程度与暴露于纯CO2环境时间呈正相关。实验组细胞倍增时间较对照组缩短。各组细胞随机运动能力无显著性差异。结论:纯CO2气体环境对卵巢癌细胞SKOV-3生长的影响与作用时间有关,卵巢癌细胞暴露于纯CO2环境短时间(1~2h)内,对细胞生长无明显影响,3h后对肿瘤细胞的生长有明显促进作用。纯CO2环境对肿瘤细胞的随机运动能力无明显影响。

Effect of carbon dioxide on growth and movement of human ovarian carcinoma cell in vitro

Objective:To observe the effects of carbon dioxide on growth and movement of human ovarian cancer cells in vitro.Such study will provide a primary evidence for the safety of CO_2 pneumoperitoneum laparoscopy surgery in malignancies.Methods:Immortalized ovarian epithelial carcinoma cell(SKOV-3 cell line) cultures were exposed to carbon dioxide.Cell growth was determined by spectrophotometric assay(MTT).Cell movement was determined with the use of Wound-assay.The results were compared with control cells by paired test and liner regressions analysis.Results:The levels of MTT of carcinoma cells that were exposed to carbon dioxide had increased after 4 days in culture.The increased cell growth had a linear relationship to the length of carbon dioxide exposure.The doubling time of carcinoma cells exposed to carbon dioxide was shorter than that in control cells.The number of migrating cells(SKOV-3)into the wound surface were no significant difference to be found among four groups(P>0.05).Conclusions:Carbon dioxide exposure increases the growth of human ovarian carcinoma cells in vitro.There is no effect on movement of SKOV-3 cell after exposed to carbon dioxide.