软肝化坚颗粒调节肝星状细胞分泌胶原及相关细胞因子的研究

目的:观察软肝化坚颗粒药物血清对肝星状细胞(HSC)分泌Ⅰ、Ⅲ型胶原及肝纤维化相关细胞因子的影响.方法:通过灌胃制备小鼠软肝化坚颗粒及秋水仙碱药物血清.将HSC-T6细胞分为3组,分别加入含10％软肝化坚颗粒血清、10％秋水仙碱血清和10％正常小鼠血清的培养基,培养24小时后,收集培养上清.采用ELISA法检测Ⅰ型胶原、转化生长因子β1 (TGF-β1)、瘦素(LP)及血小板衍生生长因子(PDGF)的含量；采用放射免疫法检测Ⅲ型胶原含量.结果:与正常血清对照组相比,秋水仙碱血清组和软肝化坚颗粒血清组培养上清中Ⅰ型胶原含量均显著降低,P值均为0.000；Ⅲ型胶原的含量也均显著降低,P值分别为0.005和0.001.与正常血清对照组相比,秋水仙碱血清组培养上清中TGF-β1、LP和PDGF的含量均明显降低,P值均为0.000；软肝化坚血清组也均显著降低,P值均为0.000；软肝化坚颗粒血清组与秋水仙碱血清组相比,培养上清中TGF-β1、LP的含量均明显降低,P值分别为0.006和0.043.结论:软肝化坚颗粒可抑制活化的HSC产生TGF-β1、PDGF及LP等细胞因子,从而对HSC分泌胶原产生抑制作用.

Influence of Ruangan Huajian granule on excretion of collagens and cytokines by hepatic stellate cells

Objective:To study the effect of Ruangan Huajian granule medicated serum on excretion of typeⅠ,Ⅲ collagen and cytokines related with hepatic fibrosis by hepatic stellate cells(HSCs).Methods:Mouse serum was prepared with Ruangan Huajian granule by gastrogarage.HSC-T6 were divided into three groups: control group incubated with normal mouse serum;Ruangan Huajian granule group incubated with Ruangan Huajian granule mouse serum;colchicine group incubated with colchicine mouse serum.Cells of each group were cultured for 24 hours,and supernatant was collected.The levels of typeⅠcollagen,transforming growth factor-β1(TGF-β1),leptin(LP) and platelet-derived growth factor(PDGF) in supernatant were analyzed by ELISA.The level of type Ⅲ collagen was analyzed by radioimmunoassay.Results:The levels of typeⅠcollagen in Ruangan Huajian granule group and colchicine group were notably lower than those in control group,P value all was 0.000.The levels of type Ⅲ collagen in Ruangan Huajian granule group and colchicine group were also significantly lower than those in control group,P value was 0.005 and 0.001,respectively.The levels of TGF-β1,LP and PDGF in Ruangan Huajian granule group and colchicine group were notably lower than those in control group,P value all was 0.000.The levels of TGF-β1 and LP in Ruangan Huajian granule group were notably lower than those in colchicine group,P value was 0.006 and 0.043,respectively.Conclusion:Ruangan Huajian granule can inhibit the excretion of TGF-β1,LP and PDGF by HSC-T6,so it can inhibit the production of typeⅠand Ⅲ collagen by HSC-T6.