重组BCG—Sj26GST疫苗诱导小鼠sIL—2R和IFN—γ的变化

目的研究日本血吸虫重组 BCG- Sj2 6 GST疫苗对小鼠脾细胞 s IL- 2 R和 IFN- γ的影响。方法第 1次实验采用 1× 10 6 和 1× 10 8CFU疫苗皮下免疫 BAL B/ C鼠 ,免疫后 8周用日本血吸虫尾蚴进行攻击感染 ,感染后 6周剖杀小鼠 ,同时设有 PBS对照组。第 2次实验用 1× 10 6 CFU疫苗皮下和静脉注射分别免疫小鼠 ,于免疫后 0、4、8、10、14和 16周各剖杀 4只 ,分离脾脏 ,用 Sj2 6或 Con A刺激脾细胞 ,用 EL ISA法检测脾细胞上清液中 s IL 2 - R和 IFN -γ含量。结果疫苗免疫 ,尾蚴攻击后 ,s IL- 2 R和 IFN- γ水平显著升高 ;动态观察发现 s IL- 2 R和 IFN- γ分别于免疫后 8～ 10周和 4～ 8周达最高水平。结论日本血吸虫重组 BCG- Sj2 6 GST疫苗可加强宿主 Th1反应 ,促进 Th1细胞分泌 IL- 2和 IFN- γ,它们与免疫细胞相互作用 ,提高宿主抗血吸虫感染的保护力

Changes of sIL-2R and IFN-

Objective To investigate the effects of recombinant BCG-Sj26GSTvaccine of schistosoma japonicum on sIL-2R and IFN-γ produced by spleen cells of mice. Methods In the first experiment， BALB／C mice were immunized subcutaneously by 1×106 and 1×108 CFU vaccine respectively， challenged with Sj cercariae 8 weeks after immunization and killed 6 weeks after infection to separate spleens， PBS served as control； in the second experiment， after immunized subcutaneously and intravenously by 1×106 CFU vaccine respectively，4 mice were killed to separate spleens on 0、4、8、10、14 and 16 weeks after immunization， spleen cells were stimulated by Sj26 or ConA. Levels of sIL-2R and IFN-γ in supernatant of spleen cells were detected by ELISA.Results Levels of sIL-2R and IFN-γ increased obviously after immunization with the vaccine against challenge with Sj cercariae； dynamic observation showed sIL-2R and IFN-γ reached the highest level on 8－10 weeks and 4－8 weeks after immunization respectively. Conclusion Recombinant BCG-Sj26GST vaccine of schistosoma japonicum may strengthen host’s Th1 response， promote Th1 cells to secrete IL-2 and IFN-γ. These cytokines and immune cells may influence each other to increase host’s protection against schistosomiasis.