丙泊酚与咪达唑仑合用对小鼠学习记忆的影响

目的:观察咪达唑仑和丙泊酚合用对小鼠学习记忆的影响。方法:小鼠分为5组(n=10):生理盐水(NS,皮下注射)组、10%脂肪乳(LM,腹腔注射)组、咪达唑仑(MZ,皮下注射1mg·kg-1)组、丙泊酚(PP,腹腔注射20mg·kg-1)组及咪达唑仑与丙泊酚合用(MP,0.5mg·kg-1+10mg·kg-1)组。用跳台和避暗实验分别观察各组不同时间相应的潜伏期、错误次数,以其评估对小鼠学习记忆的影响。结果:用药后第1、2天,MP、PP和MZ组与对照组(NS和LM)比较,跳台和避暗实验中错误次数、潜伏期均有显著性差异(P<0.01);用药后第3、4天,各组间比较各指标无显著性差异(P>0.05)。用药后第2天,与MZ组比较,MP和PP组跳台和避暗实验的错误次数减少、潜伏期延长(P<0.01),MP与PP组间比较无显著性差异(P>0.05)。结论:咪达唑仑和丙泊酚减半合用与两药单用同样可致小鼠学习记忆功能减退,二者具有协同作用。

Effect of Combination of Propofol and Midazolam on Learning Memory of Mice

OBJECTIVE： To observe the effect of the combination of propofol and midazolam on learning memory of mice. METHODS： Fifty mice were divided into 5 groups（n= 10）： NS group（normal saline, subcutaneously）, LM group（10% intralipid, peritoneal）, MZ group （ 1 mg. kg ^-1 midazolam, subcutaneously）, PP group （20 mg. kg^-1 propofol, peritoneal） and MP group （combination of 0.5 mg. kg^-1 midazolam and 10 mg. kg^-1 propofol）. Latency and error times in each group were observed by step-down test and step-through test so as to evaluate the effect of medicine on learning memory of mice. RESULTS： On the 1st and 2nd day after medication, significant differences were noted in error times and latency in MP group, PP group and MZ group, as compared with NS group and LM group（P〈0.01 ）. There was no difference among each group on the 3rd or 4th day after medication （P〉0.05）. Compared with MZ group, reduction of error times and prolongation of latency were noted in MP group and PP group on the 2nd day after medication （P〈0.01） while there was no difference between MP group and PP group （P〉0.05）. CONCLUSION： Combination of propofol and midazolam （half dose） can result in hypofunction of learning memory of mice as well as they are used alone. Synergistic action is performed between propofol and midazolam.