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Labeling of sarcoma associated monoclonal antibody with 111In, 67Ga and 125I
Authors:E Lavie  M Boazi  J Weininger  M Bitton  G Yosilevski  D Front  Y Hirshaut  E Robinson  A H Bartal
Institution:1. Key Lab of Biomedical Engineering and Shenzhen Key lab of Translational Medicine of Tumor, School of Medicine, Shenzhen University, Shenzhen, China;2. School of Electrical and Electronic Engineering, Nanyang Technological University, Singapore;3. Key Laboratory of Optoelectronics Devices and Systems of Ministry of Education/Guangdong Province, College of Optoelectronic Engineering, Shenzhen University, Shenzhen, P. R. China;1. Istituto di Chimica dei Composti OrganoMetallici - Consiglio Nazionale delle Ricerche (ICCOM - CNR), SS Pisa, Via Moruzzi 1, 56124 Pisa, Italy;2. Istituto di Chimica dei Composti OrganoMetallici - Consiglio Nazionale delle Ricerche (ICCOM - CNR), Via Madonna del Piano 10, 50019 Sesto Fiorentino (FI), Italy;3. Dipartimento di Ingegneria Civile e Industriale, Università di Pisa, Via Diotisalvi 2, 56122 Pisa, Italy;1. The Nethersole School of Nursing, Faculty of Medicine, The Chinese University of Hong Kong, Hong Kong Special Administrative Region, China;2. Nursing Department, Shijiazhuang People''s Hospital, Shijiazhuang, Hebei Province, China;3. Department of Rehabilitation Medicine, Affiliated Hospital of Hebei University, Baoding, Hebei Province, China;4. Nursing Department, The Fourth Hospital of Hebei Medical University, Shijiazhuang, Hebei Province, China
Abstract:Labeling of human sarcoma-associated murine monoclonal antibody (MAb) 23H7 with 67Ga and 111In by the bifunctional ligand method is reported. 67Ga was chelated to the MAb via desferrioxamine B and 111In via the cyclic anhydride of DTPA. Higher specific activity was obtained with 67Ga (4-5 microCi/micrograms) as compared with 111In (2 microCi/micrograms). The binding capacity of the MAb was confirmed by repeated indirect immuno-fluorescence assays performed before and after labeling. A fast blood clearance was observed: 33% recovered dose (R.D.) blood level 3 h post-injection as compared with 56% after injection of control polyclonal IgG. Preliminary results on chemically induced sarcoma bearing mice showed a relatively high tumor uptake of the labeled antibody.
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