| 柴胡皂甙d上调HL-60细胞糖皮质激素受体mRNA并诱导细胞凋亡 |
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| 引用本文： | 步世忠,许金廉,孙继虎.柴胡皂甙d上调HL-60细胞糖皮质激素受体mRNA并诱导细胞凋亡[J].中华血液学杂志,1999,20(7):354-356. |
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| 作者姓名： | 步世忠 许金廉 孙继虎 |
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| 作者单位： | 第二军医大学生理教研室(步世忠!200433上海，许金廉!200433上海，孙继虎!200433上海，陈宜张!200433上海)，中国科学院上海生物化学研究所分子生物学国家重点实验室(商权) |
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| 基金项目： | 国家中医药管理局基金!(96F0 1 6)资助 |
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| 摘 要： | 目的 观察柴胡皂甙d(SSd)对HL 6 0细胞糖皮质激素受体 (GR)mRNA表达的调节作用以及对细胞凋亡的影响。方法 选用从柴胡中提取的单体成分SSd ,以3 H 胸腺嘧啶掺入法观察SSd对细胞生长的抑制作用 ,DNA凝胶电泳及流式细胞术分析细胞凋亡 ,用Northernblot分析GRmRNA的改变。结果 经SSd作用后 ,HL 6 0细胞3 H 胸腺嘧啶掺入率明显降低 ,呈时间和剂量依赖关系 ,10 μg/mlSSd处理 48小时作用最明显 ,6 0小时时出现典型的DNA片段梯形带 ,流式细胞仪分析细胞阻滞于G1期并出现凋亡峰 ;GRmRNA表达增加。结论 SSd可上调HL 6 0细胞GRmRNA表达并诱导细胞凋亡。
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| 关 键 词： | 柴胡 受体 糖皮质激素 RNA 信使 细胞系 HL-60 细胞凋亡 |
Saikosaponin d up regulates GR mRNA expression and induces apoptosis in HL 60 cells |
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| BU Shizhong,XU Jinlian,SUN Jihu,et al.Saikosaponin d up regulates GR mRNA expression and induces apoptosis in HL 60 cells[J].Chinese Journal of Hematology,1999,20(7):354-356. |
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| Authors: | BU Shizhong XU Jinlian SUN Jihu |
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| Institution: | Department of Physiology, Second Military Medical University, Shanghai 200433. |
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| Abstract: | OBJECTIVE: To research the effect of saikosaponin-d (SSd) on the expression of glucocorticoid receptor (GR) mRNA and induction of apoptosis in HL-60 cells. METHODS: Antiproliferation effects of SSd on HL-60 cells were determined by 3H-thymidine incorporation. Apoptosis of the SSd-treated cells was determined by DNA fragmentation analysis and flow cytometry. GR mRNA was analyzed by Northern blot analysis. RESULTS: After 48 h treatment with 10 micrograms/ml SSd, 3H-thymidine incorporation was decreased in HL-60 cells and the effects were time and dose dependent. DNA ladder appeared after 60 h treatment with SSd. The flow cytometry analysis showed HL-60 cells was arrested at G0/G1 phase and apoptotic peak appeared in sub-G1 phase. The expression of GR mRNA was increased. CONCLUSION: SSd up-regulates the expression of GR mRNA, inhibits the cell growth and induces apoptosis in HL-60 cells. |
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| Keywords: | Bupleurum chinense Receptor glucocorticoid mRNA Cell lines HL 60 Apoptosis |
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