Fas基因在rhG-CSF诱导白血病细胞凋亡过程中的作用

目的探讨了Ｆａｓ基因是否参与重组人粒细胞集落刺激因子（ｒｈＧＣＳＦ）对白血病细胞的诱导凋亡过程。方法利用脂质体（ＤＯＴＡＰ）介导的基因转染技术将Ｆａｓ基因转入ＨＬ６０细胞中，并经原位杂交、Ｗｅｓｔｅｒｎｂｌｏｔ及流式细胞术（ＦＣＭ）证实已成功建立了Ｆａｓ高表达的ＨＬ６０细胞株，用ＦＣＭ对Ｆａｓ基因在ｒｈＧＣＳＦ诱导白血病细胞凋亡过程中的作用进行了探讨。结果相同浓度的ｒｈＧＣＳＦ作用相同时间后转染的ＨＬ６０细胞凋亡率较未转染的ＨＬ６０细胞凋亡率明显增加，且随ｒｈＧＣＳＦ作用时间延长，Ｆａｓ在ＨＬ６０细胞中的表达逐渐增加。结论Ｆａｓ基因是凋亡诱导基因，ｒｈＧＣＳＦ诱导白血病细胞凋亡依赖Ｆａｓ径路传递凋亡信号。

Transfection of Fas(CD95) gene into HL60 cell and its role in rhGCSFinduced apoptosis

Objective To explore the role of Fas gene in rhGCSFinduced apoptosis. Methods Mediated by lipofection (DOTAP), Fas cDNA was transferred into HL60 cells. In situ hybridization, Western blotting and FCM analysis were used to demonstrate the successful transfection. The apoptosis percentages of HL60 and Fas transfected HL60 cells were compared after coculturing with rhGCSF at the same final concentration. Results After cocultured with rhGCSF (10ng/ml) for 96 hours, the apoptosis percentage of Fas transferred HL60 cells was significantly higher than that of HL60 cells, and the Fas expressions could be upregulated by rhGCSF. Conclusions Fas is an apoptosisinduction oncogene,and rhGCSF induces apoptosis of HL60 cells through Fas signalling pathway.