血小板第4因子及四肽AcSDKP的造血保护作用研究

目的了解血小板第４因子（ＰＦ４）和四肽Ｎ乙酰丝天赖脯（ＡｃＳＤＫＰ）对５氟尿嘧啶（５ＦＵ）处理后小鼠体内造血的作用。方法实验鼠用ＰＦ４（４０μｇ／ｋｇ）或ＡｃＳＤＫＰ（４μｇ／ｋｇ）注射２次，间隔６小时，第２次注射后２０小时再注射５ＦＵ（１５０ｍｇ／ｋｇ）。第６，８和１３天时，检查高增殖潜能的集落形成细胞（ＨＰＰＣＦＣ）、红系爆式集落形成单位（ＢＦＵＥ）、粒巨噬系集落形成单位（ＣＦＵＧＭ）、巨核系集落形成单位（ＣＦＵＭＫ）及巨核细胞（ＭＫ）。结果ＰＦ４或ＡｃＳＤＫＰ可促使６～８天的ＨＰＰＣＦＣ以及８天的ＢＦＵＥ和ＣＦＵＧＭ明显增加；ＰＦ４还能促进８天的ＣＦＵＭＫ和ＭＫ增加，但ＡｃＳＤＫＰ则无此作用。结论ＰＦ４和ＡｃＳＤＫＰ虽然对巨核祖细胞的作用不同，但它们均能加速体内ＨＰＰＣＦＣ、ＣＦＵＧＭ和ＢＦＵＥ的恢复，这两种分子有可能具有保护造血细胞抵抗化疗药物杀伤的作用。

The hemoprotective effect of platelet factor 4 (PF4) and tetrapeptide AcSDKP

OBJECTIVE: To study the effects of platelet factor 4(PF4) and tetrapeptide N-acetyl-Ser-Asp-Lys-Pro(AcSDKP) on hemopoietic progenitors in mice treated with 5-Fluorouracil (5-FU). METHODS: Mice were injected with PF4 (40 micrograms/kg) or AcSDKP (4 micrograms/kg) twice at 6 h intervals, and 20 h after the second injection they were given one injection of 5-FU (150 mg/kg), and the high proliferative potential-colony forming cell (HPP-CFC), burst-forming unit erythroid (BFU-E), colony forming unit megakaryocyte (CFU-MK), and megakaryocyte (MK) were examined 6, 8 and 13 days later. RESULTS: The administration of PF4 or AcSDKP resulted in a significant increase of the number of HPP-CFC on days 6-8 and BFU-E and CFU-GM on day 8 when compared to 5-FU alone. Furthermore, PF4 was found to increase significantly the number of CFU-MK and MK on day 8, which was not observed with AcSDKP. CONCLUSION: PF4 or AcSDKP accelerate the recovery in vivo of HPP-CFC, CFU-GM and BFU-E after 5-FU treatment but their effect may be different on megakaryocytic progenitors. Both molecules may have a hemoprotective effect against chemotherapeutic agents.