再生障碍性贫血患者骨髓造血干/祖细胞c-kit受体表达

目的 观察再生障碍性贫血（再障）患骨髓造血干／祖细胞ｃ－ｋｉｔ受体（ＣＤ１１７）的表达，以了解再障碍造血功能衰竭与ｃ－ｋｉｔ受体水平间的关系。方法 采用免疫荧光法及流式细胞技术检测２０例再障患骨髓单个核细胞（ＭＮＣ）和ＣＤ３４细胞ＣＤ１１７的表达水平，结果 （１）ＭＮＣＣＤ１１７表达率慢性再障（ＣＡＡ）组为（２．０８±１．９６）％，重型再障（ＳＡＡ）组为（０．８９±０．３２）％，正常对照组和增

Expression of c kit receptor on the hemopoietic stem/precursor cells of aplastic anemia patients

OBJECTIVE: To explore the expression of c-kit receptor(CD117) on the hemopoietic stem/precursor cells of aplastic anemia patients and its role in the genesis of aplastic hematopoiesis failure. METHODS: c-kit expression on the bone marrow mononuclear cells(MNC) and CD34 positive cells from 11 chronic aplastic anemia (CAA), 9 severe aplastic anemia(SAA), 10 non-aplastic anemia(NAA) patients (5 iron deficiency anemia and 5 autoimmune hemolytic anemia) and 5 healthy controls was measured with flow cytometric immunofluorescence assay and anti-CD117 monoclonal antibody. RESULTS: The CD117 positive rates of MNC in CAA, SAA, NAA and control groups were (2.08 +/- 1.96)%, (0.89 +/- 0.32)%, (0.67 +/- 0.39)% and (0.45 +/- 0.16)%, respectively, and the rates were significantly higher in CAA and SAA groups than in NAA and control groups(P < 0.05). The CD117 and CD34 double positive cells of MNC in CAA, SAA, NAA and control groups were (0.87 +/- 0.57)%, (0.65 +/- 0.41)%, (0.46 +/- 0.26)% and (0.41 +/- 0.15)%, respectively, and were significantly higher in CAA and SAA groups than in NAA and control groups (P < 0.05). The CD117 positive cells of CD34 positive cells were also higher in CAA[(71.6 +/- 12.4)%] and SAA[(88.9 +/- 23.5)%] groups than in NAA[(41.8 +/- 10.3)%] and control [(46.3 +/- 9.7)%] groups (P < 0.01). CONCLUSION: Hemopoietic stem/precursor cells of aplastic anemia patients expressed more c-kit receptors than those of NAA patients and healthy controls. The hematopoiesis failure occurred in aplastic anemia is not attributed to low expression of c-kit receptor on the hemopoietic stem/precursor cells.