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再生障碍性贫血患者骨髓造血干/祖细胞c-kit受体表达
引用本文:邵宗鸿,陈桂彬,张泓. 再生障碍性贫血患者骨髓造血干/祖细胞c-kit受体表达[J]. 中华血液学杂志, 1999, 20(10): 532-534
作者姓名:邵宗鸿  陈桂彬  张泓
作者单位:中国医学科学院
摘    要:目的 观察再生障碍性贫血(再障)患骨髓造血干/祖细胞c-kit受体(CD117)的表达,以了解再障碍造血功能衰竭与c-kit受体水平间的关系。方法 采用免疫荧光法及流式细胞技术检测20例再障患骨髓单个核细胞(MNC)和CD34细胞CD117的表达水平,结果 (1)MNCCD117表达率慢性再障(CAA)组为(2.08±1.96)%,重型再障(SAA)组为(0.89±0.32)%,正常对照组和增

关 键 词:贫血  再生障碍性  造血干细胞  受体  ckit

Expression of c kit receptor on the hemopoietic stem/precursor cells of aplastic anemia patients
Z Shao,G Chen,H Zhang. Expression of c kit receptor on the hemopoietic stem/precursor cells of aplastic anemia patients[J]. Chinese Journal of Hematology, 1999, 20(10): 532-534
Authors:Z Shao  G Chen  H Zhang
Affiliation:Institute of Hematology and Blood Diseases Hospital, CAMS, PUMC, Tianjin 300020.
Abstract:OBJECTIVE: To explore the expression of c-kit receptor(CD117) on the hemopoietic stem/precursor cells of aplastic anemia patients and its role in the genesis of aplastic hematopoiesis failure. METHODS: c-kit expression on the bone marrow mononuclear cells(MNC) and CD34 positive cells from 11 chronic aplastic anemia (CAA), 9 severe aplastic anemia(SAA), 10 non-aplastic anemia(NAA) patients (5 iron deficiency anemia and 5 autoimmune hemolytic anemia) and 5 healthy controls was measured with flow cytometric immunofluorescence assay and anti-CD117 monoclonal antibody. RESULTS: The CD117 positive rates of MNC in CAA, SAA, NAA and control groups were (2.08 +/- 1.96)%, (0.89 +/- 0.32)%, (0.67 +/- 0.39)% and (0.45 +/- 0.16)%, respectively, and the rates were significantly higher in CAA and SAA groups than in NAA and control groups(P < 0.05). The CD117 and CD34 double positive cells of MNC in CAA, SAA, NAA and control groups were (0.87 +/- 0.57)%, (0.65 +/- 0.41)%, (0.46 +/- 0.26)% and (0.41 +/- 0.15)%, respectively, and were significantly higher in CAA and SAA groups than in NAA and control groups (P < 0.05). The CD117 positive cells of CD34 positive cells were also higher in CAA[(71.6 +/- 12.4)%] and SAA[(88.9 +/- 23.5)%] groups than in NAA[(41.8 +/- 10.3)%] and control [(46.3 +/- 9.7)%] groups (P < 0.01). CONCLUSION: Hemopoietic stem/precursor cells of aplastic anemia patients expressed more c-kit receptors than those of NAA patients and healthy controls. The hematopoiesis failure occurred in aplastic anemia is not attributed to low expression of c-kit receptor on the hemopoietic stem/precursor cells.
Keywords:Anemia  aplastic Hematopoietic stem cells Receptor  c kit
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