IL—2基因修饰增强白血病抗原冲击的树突状细胞诱导的抗肿瘤？…

目的 研究腺病毒介导的白细胞介素（ＩＬ）２基因修饰能否使抗原冲击的树突状细胞（ＤＣ）在体内诱导出更强的抗肿瘤免疫反应。方法 用小鼠粒－巨噬细胞击落刺激因子（ｍＧＭ－ＣＳＦ）和ｍＩＬ－４扩增上鼠骨髓ＤＣ在ＦＢＬ－３红白血病细胞冻融抗原冲击的同时转染ＩＬ－２腺病毒。观察其体外分泌ＩＬ－２的水平，刺激Ｔ细胞增殖的能力，其体内皮下免疫后小鼠引流淋巴结细胞数和组分的变化以及主艉地产生细胞毒性Ｔ淋巴细胞（ＣＴ

Induction of potent antitumor immune response of leukemia antigen-pulsed dendritic cells by IL-2 gene modification]

OBJECTIVE: To investigate whether adenovirus-mediated IL-2 gene modification can improve antitumor immunity of the tumor antigen-pulsed dendritic cells(DC) in vivo. METHODS: DC were generated from bone marrow, then, pulsed with FBL-3 erythroleukemia cell lysates and transfected with IL-2 gene simultaneously. IL-2 secretion and T cell stimulating activity of the DC were detected. The number and cell subsets of draining lymph nodes, cytotoxic lymphocyte(CTL) activity, and the increase of protective effect for the mice s.c. vaccinated with DC were observed. RESULTS: IL-2 gene-modified, antigen-pulsed DC could secrete high level of IL-2 in vitro, stimulate proliferation of syngeneic T cells markedly. DC vaccine could increase the number of draining lymph node and induce CTL activity, which directed to FBL-3, more significantly. After the DC vaccination, the survival time of the mice challenged with wild-type FBL-3 cells were prolonged. CONCLUSION: Antitumor immune response could be induced more potently by vaccination with IL-2 gene modified, antigen-pulsed DC.