东茛菪碱对大鼠弥漫性脑损伤的脑保护作用研究

目的:探讨东莨菪碱对大鼠弥漫性脑损伤的脑保护作用。方法:在Marmarou大鼠弥漫性脑损伤模型基础上,设置正常对照组、外伤对照组及伤后治疗组,于伤后1小时给予治疗组静脉注射氢溴酸东莨菪碱0.2 5mg/kg,测量伤后2 4小时脑组织含水量、脑组织脂质过氧化物变化、血浆纤维蛋白原含量的变化,并通过显微镜观察组织形态学的差异。结果:外伤对照组脑组织含水量较正常对照组明显增加(P <0.0 1 ) ,外伤后治疗组脑组织含水量亦较正常对照组多(P <0.0 1 ) ,但比外伤对照组明显减少(P <0.0 1 )。外伤对照组脑组织内脂质过氧化物含量较正常对照组明显增多(P <0.0 1 ) ,伤后治疗组脑组织内脂质过氧化物含量亦较正常对照组多(P <0.0 1 ) ,但较外伤对照组明显减少(P <0.0 1 )。外伤对照组血浆纤维蛋白原含量较正常对照组明显增多(P <0.0 1 ) ,伤后治疗组血浆纤维蛋白原含量较正常对照组亦较多(P <0.0 5) ,但较外伤对照组明显减少(P <0.0 1 )。镜下观察脑组织切片,可见外伤对照组脑内毛细血管大量扩张淤血,血栓形成,而伤后治疗组毛细血管扩张淤血及血栓形成明显减少。结论:东莨菪碱对大鼠弥漫性脑损伤具有脑保护作用

The research of neuro protection effect of scopolamine hydrobromide on diffuse brain injury in rats.

Objective:To study the mechanism of neuro profection effect of scopolamine hydrobromide on diffuse brain injury in rats.Methods:On the base of Marmarou,to set up control group, trauma group and treatment group.The trauma group and treatment group were treated seperately with 0.9%natrilchloridii and scopolamine hydrobromide (0.25 mg/kg) equal in volume 1 hour after brain injury,to measure the water,lipid peroxide content in brain and the fibrinogen content in blood plasma 24 hour after brain injury,and to observe the histological variations with microscope.Results:The water content in brain tissue and the fibrinogen in blood plasma of trauma group are significant higher than those of control group(P<0.01),and those of treatment group are significant higher than those of control group(P<0.01)too,but which are significant lower than those of trauma group(P<0.01).We find the histological change are significant among control group trauma group and treatment group by microscopy.Conclusions:Scopolamine hydrobromide has some neuroprotective effects on diffuse brain injury in rates.