On the accumulation of alloxan in the pancreatic β-cells |
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Authors: | Lars Hammarström Bo Hellman Sven Ullberg |
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Institution: | (1) Department of Pharmacology, Royal Veterinary College, Stockholm;(2) Department of Histology, University of Umeå, Umeå, Sweden |
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Abstract: | Summary Autoradiographic studies revealed that the radioactivity in the pancreatic islets was higher than in any other mouse tissue after intravenous injections of tracer doses of 14C-2-alloxan. The concentration of radioactivity in the endocrine pancreas concerned a great majority of the cells indicating that at least cells were involved. The uptake of the radioisotope in the pancreatic islets was considerably reduced when the small amounts of 14C-2-alloxan were complemented with carrier to bring up the total dose to the diabetogenic level or were proceeded by higher doses of non-radioactive alloxan. There was no accumulation of radioactivity in the islets after injection of the non-diabetogenic conversion products of 14C-2-alloxan obtained in an alkaline medium and only insignificant uptake was noted after exposure of the radioactive alloxan to the reactive SH-groups of glutathione. The absence of significant radioactivity in the islets of growing animals after tracer doses of 14C-2-alloxan suggests that the ability of the cells to concentrate alloxan is confined to the adult age.This study was supported by grants from the Swedish Medical Research Council, the United States Public Health Service (AM-05759-05) and Knut and Alice Wallenbergs Stiftelse. |
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Keywords: | pancreatic islets alloxan alloxanic acid diabetes autoradiography specific uptake mice rats |
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