| 3,4—二氯苯丙烯酰另丁胺对分离的神经细胞内游离钙离子的影响 |
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| 引用本文： | 王迪 陶成. 3,4—二氯苯丙烯酰另丁胺对分离的神经细胞内游离钙离子的影响[J]. 中国药学杂志, 1999, 34(6): 372-374 |
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| 作者姓名： | 王迪 陶成 |
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| 作者单位： | 锦州医学院药理教研室(王迪),北京医科大学基础医学院药理学系(陶成),锦州市中心医院麻醉科(李晓东) |
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| 摘 要： | 目的 研究新的桂皮酰胺类化合物,３,４－二氯苯丙烯酰另丁胺（简称ＡＥＤ８８０１）对分离的神经细胞内游离钙离子的影响,方法 采用Ｃａ＾２＋指示剂Ｆｕｒａ－２作为细胞钙离子的荧光探针,利用ＲＦ－５０００型双波长荧光分光光度计检测分离的神经细胞内游离钙离子及其变化,并观测ＡＥＤ８８０１对静息态细胞内钙离子,高Ｋ＾＋去极化状态及ＮＭＤＡ刺激情况下的细胞内钙离子变化的影响。结果 在卢状态下,细胞内钙离子浓度
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| 关 键 词： | 神经细胞 钙离子 脑缺血 AED8801 |
Effects of 3,4dichlorophenylpropenoylsec.butylamine on the intracellular free calcium in rat isolated brain cells |
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| Wang Di. Effects of 3,4dichlorophenylpropenoylsec.butylamine on the intracellular free calcium in rat isolated brain cells[J]. Chinese Pharmaceutical Journal, 1999, 34(6): 372-374 |
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| Authors: | Wang Di |
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| Abstract: | OBJECTIVE: To investigate the influence of 3,4dichlorophenylsec.butylamine (AED8801),a new derivative of cinnamamides on the intracellular free calcium in rat isolated brain cells. METHOD: Using Ca2 sensitive fluorescent indicator,Furo2 and a fluorescence detector (Shimadzu RF5000,Japan),intracellular free Ca2 (]i) was measured.The influence of AED8801 on the i resting status,depolarization induced by KCl and the addition of NmethylDaspartate (NMDA) were observed. RESULTS: The average intracellular free Ca2 concentration in resting status was 206.5 11.2 molL-1,depolarization induced by KCl and NMDA can increase i respectively.3,4dichlorophenylpropenoylsec.butylamine 1,10 and 100molL-1 decreased the resting status i,but no statistic significance (P>0.05) inhibited the NMDAinduced i elevation (P<0.05,P<0.01),and did not decrease the high extracellular K induced 2 ]i elevation (P>0.05). CONCLUSION: AED8801 can inhibit the Ca2 channel of receptordependent,but can not inhibit the Ca2 channel of voltagedependent. |
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| Keywords: | dichlorophenylpropenoylsec.butylamine intracellular free Ca2 concentration cerebral ischemia injury |
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