血小板受体特异性微泡造影剂靶向粘附血栓的实验研究

目的构建能够靶向血小板膜糖蛋白Ⅱb／Ⅲa受体的特异性脂质微泡，观察其对微血栓、体外人工血栓的粘附效应。方法用RGDS(Arg-Gly-Asp-Ser)耦联的方法构建靶向微泡，观察其对血小板聚集形成的微血栓的粘附效应。另在体外制作人工血栓模型，在二维超声下观察微泡造影剂对声学信号的影响。结果血小板聚集后加入经构建的靶向微泡，最后粘附在血小板微血栓周围；而加入普通微泡的对照孔则未见这种粘附效应。体外血栓模型中，未加微泡的血栓超声图像周围边界模糊，加入靶向微泡后血栓图像边界明显增强。结论血小板受体特异性靶向微泡对血栓有明显的趋附作用．并且能够增强血栓的超声信噪比。对血栓的临床诊断和治疗有潜在的意义。

Targeted adhesion of platelet receptor-specific microbubble agent to thrombus in vitro]

OBJECTIVE: To examine the thrombus-targeting effect of platelet receptor-specific lipid microbubbles. METHODS: The targeted microbubbles were prepared by coupling Arg-Gly-Asp-Ser (RGDS) with the lipid microbubbles, which were added to the microthrombus generated by platelet aggregation. The effects of the targeted microbubbles on the ultrasonic signal was observed in an artificial thrombus model. RESULTS: The targeted microbubbles were adhesive to the microthrombus, while the non-targeted microbubbles did not possess this property. The ultrasonic signal of the thrombus border was enhanced significantly after the addition of the targeted microbubbles. CONCLUSIONS: Platelet receptor-specific microbubbles possess significant adhesive property to the thrombus and can improve signal-to-noise ratio of the thrombus, suggesting the potential value of the targeted microbubbles for clinical application in the diagnosis and treatment of thrombus.