Bc1-2家族是人参皂甙Rg1抗黑质神经元凋亡的重要调控蛋白

目的　探讨Bc1 2、Bc1 x1和Bax蛋白在人参皂甙Rgl抗帕金森病 (PD)小鼠模型黑质神经元凋亡过程中的可能作用。　方法　PD模型组单纯以 1 甲基 4 苯基 1,2 ,3,6 四氢吡啶 (MPTP)腹腔注射C5 7BL小鼠 ;预防组预先 3d腹腔注射Rg1(2 5、5 0、10 0mg kg) ,再注射MPTP ,以Niss1染色、TH组织化学染色、TUNEL染色观察黑质神经元的变化 ;免疫组织化学染色检测Bc1 2、Bc1 x1和Bax蛋白表达。　结果　 5 0和 10 0mg kgRg1预处理PD模型鼠能使黑质致密带 (SNc)部位的Niss1和TH染色阳性神经元增多 ,TUNEL染色阳性率降低 ,以及Bc1 2和Bc1 x1表达增加 ,Bax表达减少。　结论　Rg1预处理对PD模型鼠黑质神经元凋元有明显的保护作用 ;Bc1 2和Bc1 x1表达水平升高和Bax表达水平降低可能是Rg1抗凋亡的重要机制

Bcl-2 FAMILY:THE IMPORTANT REGULATING PROTEINS DURING GINSENOSIDE RG1 PROTECTION AGAINST APOPTOSIS OF NIGRA NEURONS

Objective To explore the role of Bc1|2,Bc1|x1 and Bax during the process of Ginsenoside Rg1 protecting against apoptosis of substantia nigra neurons in Parkinson disease(PD) mouse model. Methods C57BL mice were administrated i.p. with MPTP to produce PD mouse model.And different doses of Rg1(2 5,5 0,10 0?mg/kg) were given i.p. prior 3 days to MPTP in preventive groups.Nissl staining,tyrosine hydroxythase(TH) immunostaining and TUNEL staining were used to observe the changes of nigra neurons.Bc1|2,Bc1|x1 and Bax immunostaining were used to detect the expression of these proteins. Results Pretreatment with Rg1(5 0,10 0?mg/kg) could prevent the loss of Nissl staining neurons and TH|positive neurons,and decrease TUNEL|staining ratio.Rg1 could enhance the expression level of Bc1|2 and Bc1|x1,and reduce the expression level of Bax. Conclusion Rg1 has protective effect on apoptosis of substantia nigra neurons in PD mouse model.The increased expression level of Bc1|2 and Bc1|x1,and the decreased expression level of Bax may be the important mechanism during Rg1 protection against apoptosis.