室内空气污染对哮喘儿童血清miR-155水平的影响

目的:探讨室内空气污染与儿童哮喘的关系及潜在的分子机制。方法:选择176名健康受试者作为对照组,176例哮喘患儿作为哮喘组。应用实时荧光定量PCR技术检测受试者血清miR-155水平。测量甲醛(HCHO)、NO_2和颗粒(PM_(10)、PM_(2.5)和PM_1)。通过单因素和多因素logistic回归分析,评价空气污染物与哮喘风险的关系。结果:哮喘组血清miR-155水平明显高于对照组(P0.001)。两组儿童过敏史、母乳喂养史、环境烟草烟雾史、PM_(2.5)、HCHO差异与儿童哮喘发作相关(P0.05)。哮喘组血清miR-155水平与室内PM_(2.5)、HCHO水平密切相关(P0.05),对照组无此相关性。结论:哮喘患儿血清miR-155水平与室内PM_(2.5)和HCHO水平密切相关。室内空气污染加重了西安地区儿童哮喘的发生,并诱导其miR-155水平变化。

Effects of indoor air pollution on asthma and serum level of miR-155 in children

Objective: To explore the relationship between indoor air pollution and asthma in children and its potential molecular mechanism. Methods: A total of 176 healthy subjects were selected as control group and 176 asthmatic children as asthma group. The level of serum miR-155 was detected by real-time fluorescence quantitative polymerase chain reaction (PCR). HCHO, NO₂, and particles (PM₁₀, PM_2.5, and PM₁) were measured. Univariate and multivariate logistic regression analysis were used to evaluate the relationship between air pollutants and asthma risk. Results: The serum level of miR-155 in asthma group was significantly higher than that in control group (P<0.001). Allergy history of Children, breastfeeding history, environmental tobacco smoke history, PM_2.5, and HCHO were related to the occured of asthma (P<0.05). The level of serum miR-155 in asthma group was related to the levels of PM_2.5 and HCHO in the room (P<0.05), and this relationship was not observed in the control group. Conclusions: The level of serum miR-155 is closely related to the levels of PM_2.5 and HCHO in children with asthma. Indoor air pollution aggravates the incidence of asthma in children in Xi''an province of China, and induces change of miR-155 level.