全自动微柱凝胶法用于直接抗人球蛋白试验初筛的临床观察

目的:观察全自动微柱凝胶法用于直接抗人球蛋白试验初筛的结果及假阳性标本的临床特征。方法:采用全自动微柱凝胶法对1 205例临床送检标本进行直接抗人球蛋白试验初筛,阳性结果标本采用试管法进行IgG/C3d分型。结果:在1 205例标本中检出阳性180例(阳性率14.9%),凝集强度分布为:++++6例、+++23例、++41例、+95例、+/-15例。18例初筛阳性标本试管法分型结果为阴性,所有后续试管法阴性均来自于弱阳性患者,多见于淋巴造血系统疾病及自身免疫性疾病患者中。结论:全自动微柱凝胶法用于直接抗人球蛋白试验初筛具有操作标准化、结果清晰可保存、敏感性高的优点,小部分假阳性可通过严格标本前处理质控等操作,来保证结果的可靠性。

Application of automated microcolumn gel method for clinical screening of direct antiglobulin test

Objective: To investigate the results of automated microcolumn gel method in direct antiglobulin test screening and the clinical characteristics of false positive results. Methods: A total of 1205 samples from Dec. 2016 to Nov. 2017 were included in the study. Automated microcolumn gel method was used for direct antiglobulin test screening, when the result was positive, manual tube method was used to further define the type of IgG/C3d. Results: Out of the 1 205 samples, 180 (14.9%) were positive, which were graded as follows:𠦌, 6 cases; ⫵, 23 cases; ⫲, 41 cases; +, 95 cases; +/-, 15 cases. And results of 18 cases were positive using the gel method (+, 13; +/-, 5), and were negative using the tube method and mostly were from patients with lymphatic hematopoietic system disease or autoimmune disease. Conclusions: Automated microcolumn gel method has the advantages of standardized operation, clear and preservable results and high sensitivity, but may have a certain proportion of false positives which can be excluded by strict pretreatment quality control.