Notchl和NUMB在胃癌组织中的表达水平及其意义

目的研究Notchl和NUMB在胃癌组织中的表达情况及其与胃癌临床病理特征的关系。方法采用Real—time PCR和Western blotting检测胃癌肿瘤组织和相应正常组织内Notchl和NUMB的表达。结果Notchl mRNA在胃癌中的平均表达水平是正常胃组织的1．67倍（P〈0．05），且与肿瘤的分化程度和淋巴转移有关。NUMB mRNA在胃癌中的平均表达水平仅是正常胃组织的0．597倍（P〈0．05），与肿瘤分化程度有关。Notchl和NUMB在胃癌组织中的表达呈负相关关系（r=-0．459，P〈0．05）。胃癌肿瘤组织中Notchl蛋白的相对含量为（0．348±0．133），明显高于正常组织中Notchl蛋白的相对含量（0．208±0．140）（P〈0．05），胃癌肿瘤组织中NUMB蛋白的相对含量为（0．490±0．440），明显低于正常组织中NUMB蛋白的相对含量（0．746±0．390）（P〈0．05）。结论在胃癌中存在Notch信号转导通路的过度活化，Notchl的上调和NUMB的下调在胃癌发生、发展中可能发挥重要的作用。

Expression and clinical significance of Notchl and NUMB in gastric cancer

Objective To investigate the expression of Notchl and NUMB in gastric cancer and explore the relationship between two genes and clinicopathologic features. Methods The mRNA and protein expressions of Notchl and NUMB in the gastric cancer tissues and normal gastric tissues were determined by realtime PCR and Western blotting. Results The mRNA expression of Notchl in gastric cancer tissues was 1.67 times that of Notchl in normal gastric tissues. The increase of the mRNA expression of Notchl was correlated with tumor differentiation and lymph node metastatis of the patients （ P 〈 0.05 ）. The mRNA expression of NUMB in gastric cancer tissues was 0.597 times that of Notchl in normal tissues. The decrease of the mRNA expression of NUMB was correlated with tumor differentiation of the patients （P 〈 0.05 ）. The expression of Notchl was negatively correlated to that of NUMB （ r = - 0.459, P 〈 0.05 ）. The protein expression of Notchl in gastric cancer tissues was （0. 348± 0. 133 ） , which was significantly higher than （0. 208 ±0. 140 ） in normal gastric tissues （ P 〈 0.05 ）. The protein expression of NUMB in gastric cancer tissues was （0.490 ±0. 440）, which was significantly lower than 0.746 ± 0.390 in normal gastric tissues （ P 〈 0.05 ）. Conclusion Up-regulation of Notchl and down-regulation of NUMB may play an important role in the occurrence and development of gastric cancer.