便秘大鼠结肠超微结构的改变

目的观察大鼠慢传输型便秘动物模型结肠壁超微结构,揭示慢传输型便秘的发病机制.方法利用中药大黄制作大鼠慢传输型便秘动物模型,电镜观察大鼠结肠壁形态结构的变化.结果光镜下与对照组相比,大黄组表现为粘膜慢性炎症;全层大量嗜酸性粒细胞浸润;肌层胶原纤维增生;肌间神经丛神经细胞空泡变性.电镜下大黄组与对照组相比,结肠肌间神经丛Ca-jal间质细胞消失,神经元、胶质细胞退行性变.平滑肌细胞的肌纤维结构破坏,大量嗜酸性粒细胞浸润.结论大黄组结肠壁肌间神经丛出现明显的病理改变,神经递质产生及传递障碍,是慢传输型便秘的病理基础;以大黄为代表的刺激性泻剂可损伤肠神经系统.

Colonic ultrastructural changes in rats with constipation

Objective To observe the ultrastructure of colonic wall in rat model of slow transit constipation (STC) and to find the pathogenesis of STC. Method Rhubarb was used to establish STC rat model. The ultrastructural changes of colonic wall in model were observed with the electron microscope. Result With the light microscope the chronic mucositis in colon, panmural infiltration of a large number of oxyphilic granulocytes, accrementition of muscularis collagen fibers, vacuolar degeneration of nerve cells of myenteric phexus were observed in the rhubarb group. With the electron microscope the disappearance of Cajal' cells of myenteric phexus, spondylosis of neurons and neuroglia cells, disorganization of smooth muscle fiber and infiltration of oxyphilic granulocytes were observed in the rhubarb group. Conclusion There are distinct pathologic changes of colonic myenteric plexus in STC rat model. The production and transmission interference of neurotransmitter are thought as the pathologic base of STC. Stimulant cathartic like rhubarb can injure the enteric nervous system.