食管癌bax和bcl-2蛋白的定性和定量研究

目的：探讨bax和bcl-2蛋白表达在食管癌发生和发展中的作用。方法：应用免疫组化，流式细胞术和细胞免疫荧光技术对70例食管鳞癌组织bax和bcl-2蛋白进行定性和定量检测。结果：免疫组化研究示bax 蛋白阳性表达32例，bcl-2蛋白阳性表达43例，bax和bcl-2蛋白表达与肿瘤病理分级和淋巴结转移密切相关（P＜0．05），但与临床分期之间差异无显著意义（P＞0．05），流式细胞定量研究示bax蛋白阳性表达39例,bcl-2蛋白阳性表达65例，bax和bcl-2蛋白表达率和表达量与肿瘤病理分级，分期和淋巴结转移无明显相关（P＞0．05），结论：bax和bcl-2蛋白的异常表达在食管癌发生和发展中起重要作用。

Qualitative and quantitive study of bax and bcl-2 protein expression in esophageal carcinona

Objective To explore the relationship between the abnormal Bax and Bcl 2 expression and carcinogenesis of esophageal carcinoma.Methods Bax and Bcl 2 protein expression were qualitatively and quantitatively detected using immunohistochemistry and flow cytomytry and celluar immunofluoresence technique from 70 cases of esophageal squamous cell carcinoma.Results The positive rate of Bax and Bcl 2 were 45.7% and 61.4% respectively by immunohistochemistry,Bax and Bcl 2 protein expression were well correlated with the pathological grading and lymph node metastasis(P<0.05).The positive rate of Bax and Bcl 2 were 55.7% and 92.9% respectively by flow cytomytry and celluar immunofluoresence technique,Bax and Bcl 2 protein expression were not correlated with the pathological grading and staging and lymph node metastasis(P>0.05).Conclusion Bax and Bcl 2 overexpression plays an important role in the development of esophageal carcinoma.