一氧化氮与内皮素在冠心康胶囊对实验性急性心肌缺血大鼠心肌损伤保护中的作用

目的探讨一氧化氮(NO)和内皮素(ET)在冠心康胶囊保护大鼠心肌损伤中的作用。方法将30只雄性Wistar大鼠随机分为正常对照组、急性心肌缺血大鼠心肌损伤模型组和冠心康胶囊治疗组,每组10只。实验中监测各组大鼠心电、心肌酶(CK,CK-MB,LDH)变化,同时观察血清和心肌组织匀浆中NO和ET水平的改变。结果冠心康胶囊治疗组可显著降低心肌缺血大鼠心电图ST-T段变化值,治疗组的心肌酶(CK,CK-MB,LDH)水平较模型组明显降低(P<0.01);血清和心肌组织匀浆中NO明显升高,而血清和心肌组织匀浆中ET含量明显降低(均P<0.05)。结论冠心康胶囊能明显减轻急性心肌缺血心肌损害,能提高血清及心肌组织的NO水平,并降低ET的水平;NO和ET的变化可能是其防治心肌损伤的重要机制之一。

Effects of Guanxinkang Capsules on Nitric Oxide and Endothelin in Myocardium Damaged Rats Induced by Acute Myocardial Ischemia

Objective To evaluate the role of nitric oxide(NO) and endothelin(ET) in the protective effect of Guanxinkang Capsules in myocardium damaged rats.Methods 30 male wistar rats were randomly divided into 3 groups,control group(n= 10),acute myocardial ischemia group(n = 10) and Guanxinkang Capsules group(n= 10).During the experiment, electrocardiography and serum CK,CK-MB and LDH were compared among the 3 groups.The levels of NO and ET in serum,myocardium were also measured.Results The rising degree of ST-T obviously decreased in acute myocardial ischemia rats treated with Guanxinkang Capsules.The levels of serum CK,CK-MB and LDH,all decreased compared with model group(P<0.01).In addition,the NO levels in serum and myocardium increased significantly,while the ET level in myocardium markedly decreased compared with model group(P<0.05).Conclusion Guanxinkang Capsules can effectively ameliorate the myocardium injury induced by acute myocardial ischemia increase NO and decrease ET.The changes of NO and ET may play a role in the pathogenesis and progress of myocardial injury.