| 单面针茎中氯化两面针碱和橙皮苷含量测定 |
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| 引用本文： | 韦熹苑,裴刚,袁园.单面针茎中氯化两面针碱和橙皮苷含量测定[J].中国现代药物应用,2014(3):24-25. |
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| 作者姓名： | 韦熹苑 裴刚 袁园 |
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| 作者单位： | [1]广西花红药业股份有限公司,柳州545007 [2]湖南中医药大学药学院,柳州545007 |
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| 摘 要： | 目的利用HPLC测定单面针茎中氯化两面针碱和橙皮苷的含量。方法采用AgilentZorbaxSB—C18柱（250mm×4．6mm，5μm），分别以0．02mol／ml磷酸二氢钾：乙腈（74：26）、甲醇：水（40：60）为流动相，流速均为1．0ml／min，柱温均为30℃，检测波长分别为328nm和283nm。结果氯化两面针碱，橙皮苷分别在0．072～0．36μg和0．1472～0．736μg范围内线性关系良好；平均回收率（n=5）分别为100．56％、100．86％。结论该方法为单面针药材的质量控制提供实验依据，该方法具有简便、快速、准确、重现性好的特点。
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| 关 键 词： | 单面针 氯化两面针碱 橙皮苷 HPLC |
Content determination of nitidin and hesperidin from the stem of zanthoxylum dissitum |
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| WEI Xi-yuan,PEI Gang,YUAN Yuan.Content determination of nitidin and hesperidin from the stem of zanthoxylum dissitum[J].Chinese Journal of Modern Drug Application,2014(3):24-25. |
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| Authors: | WEI Xi-yuan PEI Gang YUAN Yuan |
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| Institution: | . Guangxi Huahong Pharmaceutical Co., ltd., Liuzhou 545007, China |
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| Abstract: | Objective The content of nitidin and hesperidin in the stem of zanthoxylum dissitum was determined by HPLC. Methods Agilent Zorbax SB-C18 （250mm × 4.6mm, 5μm）was used, a mobile phase of 0.02mol/ml KH2PO4- acetonitrile（74：26）, methanol-water（40：60）, the flow rate was 1.0 ml/min, the column temperature was 30℃, the detection was done at λ =328nm and λ =283nm. Results There was good linear relationship at the ranges of 0.072-0.36 μg and 0.1472-0.736 μg for nitidin and hesperidin. The average recoveries（n=5） were 100.56% and 100.86% respectively. Conclusion This method provides experimental basis for the quality control of single needle medicine and it is simple, rapid, accurate and good reproducibility. |
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| Keywords: | Zanthoxylum dissitum Nitidin Hesperidin HPLC |
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