荧光原位杂交技术检测肺癌第6号染色体畸变

目的 探讨第6号染色体畸变与肺癌发生、发展及预后的关系。方法 应用荧光原位杂交技术（FISH）对30例肺癌、10例正常肺组织标本进行第6号染色体检测。结果 本组30例肺癌组织第6号染色体出现单体、3～7体畸变，畸变率100％（30／30）；正常肺组织第6号染色体无畸变，肺癌与正常肺组织比较，P〈0．001，差异极为显著。结论 肺癌第6号染色体畸变发生在肺癌早期，可能与肺癌的发生、发展密切相关，其改变与患者的临床分期、病理分化有一定的相关性。

Detection of Chromosome 6 Aberration in Lung Carcinoma Using Fluorescence in Situ Hybridization (FISH)

Objective To study the relationship between chromosome aberration and the pathogenesis, development and prognosis of lung carcinoma. Methods Tissue speciments from 30 cases of lung carcinoma and 10 cases of normal lung tissue were detected using FISH with chro- mosome 6 probe, respectively. Results All of the 30 cases of lung carcinoma were found chromosome 6 aberration, involved monosomy, trisomy, and even beptasomy. But there was no chromosome 6 aberration found in normal lung tissues. The significant difference was between these two groups. Conclusion Chromosome 6 aberration may occur in the early stage of lung carcinoma, which may closely relate with the process of pathogenesis, development of lung carcinoma. The chromosome 6 aberration may have the significant association with clinical phase and pathological differentiation among the patients.