雷公滕及铅、镉对小鼠睾丸间质细胞一氧化氮合酶活性的影响

目的　探讨雷公藤及铅、镉等重金属元素对睾丸间质细胞一氧化氮合酶 (NOS)活性的影响。　方法　用 NADPH- d黄递酶组织化学方法 ,观察给予氯化镉、醋酸铅、雷公藤 1、2、3、4周后 ,小鼠睾丸间质 NOS活性的变化。　结果　正常组间质细胞呈 NOS强阳性反应 ,而曲精小管生精细胞均呈 NOS阴性反应。给予雷公藤 2周内NOS反应无变化 ,第 3和第 4周后 ,NOS阳性的睾丸间质细胞数量无明显变化 ,但 NOS活性稍降低。给予醋酸铅和氯化镉 1周后 ,NOS阳性的睾丸间质细胞数量明显减少 ,并随给药时间延长而加重 ,NOS活性逐渐降低 ,给药 4周后 NOS阳性的睾丸间质细胞几乎不着色。　结论　雷公藤虽然具有明显抗生育作用 ,但对 NOS阳性的睾丸间质细胞数量及 NOS活性无明显影响 ;而铅、镉等对睾丸有毒理作用的重金属元素 ,不但可使 NOS阳性的睾丸间质细胞减少 ,NOS活性也明显降低。

EFFECTS OF TRIPTERYGIUM WILFORDII F, LEAD AND CADMIUM ON NOS ACTIVITY OF MICE TESTIS INTERSTITIAL CELLS

Objective\ To explain the effects of nitric oxide (NO) on testis interstitial cells (IC) after administration of tripterygium wilfordii f.(TWF), lead acetate and cadmium chloride. Methods\ Using NADPH\|d histochemistry to investigate activity change of NOS in the testis of mice, which were survived for 1,2,3,4 weeks after administration of TWF, lead acetate and cadmium chloride. Results\ The interstitial cells (IC) of the testis were strong NOS\|activity in the normal group, spermatogenic cells were all NOS\|negative. Two weeks after administration of TWF, The NOS\|activity had no change, during 3rd and 4th weeks NOS\|positive IC had not obvious changes in number, but NOS activity was a bit lower. One week after administration of lead acetate and cadmium chloride, the numbers of NOS\|positive IC reduced significantly. With prolonging administration of such metal salts NOS activity gradually reduced. In 4 weeks after administration of the such metal salts, there were rarely seen NOS\|positive IC, and they were almost not stained. Conclusions\ TWF may have remarkable antifertility whereas the numbers and the activity of NOS of IC are not affected by TWF. In contrast, cadmium and lead were harmful to the testis. They not only reduce the numbers of IC, but also interfere NOS activity significantly.