| 过氧化氢对K562细胞凋亡的影响 |
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| 引用本文： | 刘梅冬,肖卫民,蒋碧梅,石永忠,王慷慨,肖献忠.过氧化氢对K562细胞凋亡的影响[J].中南大学学报(医学版),2003,28(5):499-501. |
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| 作者姓名： | 刘梅冬 肖卫民 蒋碧梅 石永忠 王慷慨 肖献忠 |
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| 作者单位： | 中南大学湘雅医学院病理生理学教研室,长沙,410078 |
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| 基金项目： | 国家自然科学基金资助 ( 3 0 0 0 0 0 693 0 2 70 5 3 3 )，国家 973重点项目资助 (G2 0 0 0 0 5 690 8) |
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| 摘 要： | 目的 :探讨活性氧对K5 6 2细胞凋亡的影响。方法 :用过氧化氢 (hydrogenperoxide ,H2 O2 )作用于对数生长期的K5 6 2细胞。结果 :Hoechst 332 5 8染色法观察到K5 6 2细胞明显凋亡 ;caspase活性定量测定及WesternBlotting检测显示caspase 3, 8, 9同时被激活。结论 :H2 O2 可诱导K5 6 2细胞凋亡 ,且通过同时激活线粒体途径和死亡受体途径起作用。
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| 关 键 词： | K562细胞 过氧化氢 细胞凋亡 线粒体途径 死亡受体途径 凋亡蛋白酶 |
| 文章编号： | 1000-5625(2003)05-0499-03 |
| 修稿时间： | 2003年1月14日 |
Effect of hydrogen peroxide on the apoptosis of K562 cells |
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| LIU Mei dong,XIAO Wei min,JIANG Bi mei,et al..Effect of hydrogen peroxide on the apoptosis of K562 cells[J].Journal of Central South University (Medical Sciences)Journal of Central South University (Medical Sciences),2003,28(5):499-501. |
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| Authors: | LIU Mei dong XIAO Wei min JIANG Bi mei |
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| Institution: | (Department of Pathophysiology, Xiangya School of Medicine, Central South University, Changsha 410078, China) |
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| Abstract: | Objective To investigate the effect of reactive oxygen species on the apoptosis of K562 cells. Methods K562 cells were treated with hydrogen peroxide (H 2O 2 ) of different doses for different time. Results The apoptosis of K562 cell was observed through the Hoechst 33258 staining. The caspase activity determination and Western blotting showed that caspase 3, 8, 9 were all activated. Conclusion Hydrogen peroxide can induce K562 cell apoptosis through both mitochondria and death receptor signal pathways. |
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| Keywords: | K562 cell * hydrogen peroxide apoptosis mitochondria pathway * death receptor pathway * caspases |
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