上海市金山区2010年-2011年流感监测结果分析

目的:对金山区唯一一家哨点医院采集的流感样病例咽拭子标本进行检测,以掌握2010年-2011年度金山区流感流行情况。方法:提取咽拭子标本中的病毒核酸,采用实时荧光定量PCR检测新甲H1N1、季节性H1N1、季节性H3N2、B型流感病毒,对阳性标本进行病毒培养,并进行鉴定。结果:2010年1月-12月,共采集样品631例,阳性数189例,阳性率30.0%,培养出符合滴度的流感病毒毒株6株,为5株B Vic-toria系和1株B Yamagate系;2011年1月-2011年12月,共采集312例,阳性数81例,阳性率26.0%,培养出符合滴度的流感病毒毒株30株,包括5株季节性H3型,4株新甲型H1N1型,13株B(Victoria系)型,8株B(Yamagate系)型。结论:2010年、2011年金山区流感流行特点为冬春季和夏季高发,病毒优势株从数量上呈B、新甲H1N1、季节性H3交替流行态势。

Analysis of influenza surveillance in Jinshan district during 2010-2011

Objective:To detect throat swab specimens from influenza cases in sole sentinel hospital of Jinshan district,in order to analyze the epidemic characteristics of influenza in 2010-2011.Methods:Virus nucleic acid isolated from specimens was detected for influenza A(H1N1),seasonal H1N1,seasonal H3N2 and influenza B by real-time RT-PCR.Then the positive specimens were processed to culture the influenza virus for identification.Results:631 samples were collected in 2010,189 cases were positive and the positive rate was 30.0%.6 strains of influenza virus were cultured,5 B Victoria and a B Yamagate;312 samples were collected in 2011,81 cases were positive and the positive rate was 26.0%.30 strains of influenza virus were cultured,5 seasonal H3N2,4 H1N1,13 B Victoria and 8 B Yamagate.Conclusion:The epidemic peaks of influenza were winter-spring and summer in Jinshan area from 2010 to 2011,the dominant strains were an alternation among influenza B,H1N1 and H3N2 in number.