| 高效液相色谱法测定活血调经丸中丹皮酚含量 |
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| 引用本文： | 林冬杰,卢万飘,蔡月莲.高效液相色谱法测定活血调经丸中丹皮酚含量[J].中国药业,2010,19(16):24-25. |
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| 作者姓名： | 林冬杰 卢万飘 蔡月莲 |
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| 作者单位： | 1. 广西壮族自治区梧州食品药品检验所,广西,梧州,543002
2. 桂林医学院,广西,桂林,541000 |
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| 摘 要： | 目的建立活血调经丸中丹皮酚含量的测定方法。方法采用高效液相色谱（HPLC）法,岛津VP-ODS色谱柱（250mm×4.6mm,5μm）,流动相为甲醇-水（60∶40）,流速为1.0mL/min,检测波长为274nm。结果丹皮酚进样量在7.84～78.40μg（r=0.9999）范围内与峰面积呈良好的线性关系,平均加样回收率为99.20%,RSD=0.49%（n=9）。结论 HPLC法简便快捷、结果准确,可用于活血调经丸中丹皮酚的含量测定及该制剂的质量控制。
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| 关 键 词： | 活血调经丸 高效液相色谱法 丹皮酚 |
Determining Paeonol in Huoxue Tiaojing Pills by HPLC |
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| Lin Dongjie,Lu Wanpiao,Cai Yuelian.Determining Paeonol in Huoxue Tiaojing Pills by HPLC[J].China Pharmaceuticals,2010,19(16):24-25. |
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| Authors: | Lin Dongjie Lu Wanpiao Cai Yuelian |
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| Institution: | 1. Wuzhou Institute for Food and Drug Control, Wuzhou, Guangxi, China 543002; 2. Guilin Medical College,Guilin,Guangxi,China 541000) |
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| Abstract: | Objective To establish a HPLC method for determining the paeonol content in Huoxue Tiaojing Pills. Methods HPLC was used to detect paeonol in Huoxue Tiaojing Pills, with Shimadz VP-ODS C18 column（250 mm × 4. 6 mm, 5 μm） . The mobile phase consisted of methanol-H2O（60 ∶ 40） . The flow rate was 1. 0 mL / min. The detecting wavelength was at 274 nm. Results The method was linear at the range of 7. 84-78. 40 μg for paeonol（ r = 0. 999 9）. The average recovery rate was 99. 20% , RSD = 0. 49% （ n = 9）. Conclusion The method is simple and accurate,which can be applied to the content determination of paeonol and the quality control of Huoxue Tiaojing Pills. |
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| Keywords: | Huoxue Tiaojing Pills HPLC paeonol |
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