深低温保藏软骨细胞的胶原凝胶立体培养

目的研究经深低温保藏后软骨细胞在胶原凝胶载体中的生长特性。方法取4周新西兰兔关节软骨，经分离成软骨细胞悬液后，置于深低温保藏。2月后，复苏被冻存的软骨细胞，包埋于含小牛血清和DMEM的胶原凝胶中培养，通过组织化学和透射电镜观察进行分析。结果与新鲜软骨相同，经深低温保藏的软骨细胞，复苏后包埋在胶原凝胶中培养7d，见单个的软骨细胞形成增殖的两个或两个以上的同源细胞，细胞周围集聚异染性细胞外基质。结论经深低温保藏的软骨细胞，在胶原凝胶中培养，仍保持了分裂增殖和合成细胞外基质的能力，可作为种子细胞，供组织工程修复软骨组织。

Study Of Chondrocytes Embedded in Collagen Gel Culture after Preservation in Deep Hypothermia

Objective The growth characteristics of chondrocytes after preservation in deep hypothermia embedded in collagen gel culture were studied in vitro. Methods Chondrocytes isolated from the articular cartilage of 4 weeks old New Zealand rabbits were preserved in deep hypothermia. After 2 months, these Chondrocytes after resuscitation were dispersively embedded in the collagen gels containing DMEM medium and fetal calf serum, and were examined by histochemistry and electron microscope respectively. Results Similar to the fresh chondrocytes preserved in deep hypothermia were evidenced by homologous cytes proliferated from the original single cyte after being embedded in collagen gels for 1 week. These proliferated cytes were surrounded metachromatic extracellular matrix by deposition. Conclusion The chongdrocytes culture in collagen gels preserved in deep hypothermia remain their abilities of proliferation and excretion of extracellular matrix. These cytes, as seeds, can be used to repair the cartilage by bioengineering.