尼美舒利对结肠癌细胞株PGE2及VEGF表达的影响

目的:探讨选择性环氧化酶2(COX-2)抑制剂对COX-2高表达的结肠癌细胞株HT-29增殖和凋亡的影响,明确以COX-2为靶点治疗结肠癌的作用途径。方法:将选择性COX-2抑制剂尼美舒利(nimesulide)按不同浓度作用于结肠癌细胞系HT-29,用MTT(四甲基偶氮唑蓝比色法)法分别于0、24、48、72h检测细胞增殖状态;流式细胞仪观察尼美舒利对细胞凋亡的影响,进一步采用ELISA法检测药物作用后前列腺素E2(PGE2)的表达,免疫组化法测定内皮细胞生长因子(VEGF)表达阳性率。结果:尼美舒利对结肠癌细胞系HT-29呈时间、剂量依赖性方式抑制细胞增殖,促进其凋亡。PEG2及VEGF表达水平随作用时间延长而下降。结论:选择性COX-2抑制剂可能通过PGE2与VEGF途径影响结肠癌细胞HT-29的增殖与凋亡,是其治疗结肠癌的分子机制。

Effect of Nimesulide on the Expression of PGE2 and VEGF of Human Colon Cancer Cells

Objective:To investigate the influence of nimesulide,a selective cyclooxygenase 2(COX-2) inhibitor,on the prolif- eration and apoptosis of colorectal cancer cell HT-29,and to explore it's potential mechanism to treat colorectal carcinoma. Methods:Cultured HT-29 cells were treated with nimesulide.MTT assay and flow eytometry were used to measure the prolifer- ation and apoptosis in 0,24,48,72 h respectively.The expression of PGE2 and VEGF was detected by ELISA and immunohis- tochemistry methods separately after treatment with nimesulide.Results:Nimesulide inhibited the cells proliferation and in- duced apoptosis in a dose-and time-dependent manner,and resulted in a significant down-regulation of PGE2 and VEGF.Con- clusion:Our results showed that Nimesulide may inhibit the proliferation and induce apoptosis of colon cancer cell through de- creasing expression of PGE2 and VEGF.This may be a new interfering target of selective COX 2 inhibitor on treating colon cancer.