大鼠急性胰腺炎模型脾脏免疫状态的研究

目的 检测大鼠急性胰腺炎模型外周血及脾脏T淋巴细胞亚群，探讨急性胰腺炎病情发展过程中脾脏免疫功能状态的改变。方法 雄性SD大鼠随机分为对照组和实验组，胰管内逆行注射4％牛磺胆酸钠建立实验组胰腺炎模型，两组分别在3、6、12h分3批处死，每批12只。每只大鼠取胰腺组织观察病理改变，取股静脉血2ml及脾脏2g制成单细胞悬液，用流式细胞仪测定T淋巴细胞亚群CD3^ 、CD4^ 、CD8^ 及CD4^ ，／CD8^ 比值。结果 在胰腺组织炎性水肿阶段T淋巴细胞亚群无明显改变，在出血坏死阶段外周血CD3^ 、CD4^ 降低、CD8^ 轻度升高、CD4^ ／CD8^ 明显降低，脾脏细胞与外周血相比CD3^ 、CD4^ 及CD4^ ／CD8^ ，明显降低，脾脏呈现负性免疫状态，且随着炎症的加重变化更加明显。结论脾脏在重症急性胰腺炎中起负性免疫作用，加重SAP病情发展。

Study on immune status of spleen in rats with acute pancreatitis

Objective To examine T-lymphocyte subsets of peripheral blood and splenic cells in rat with acute pancreatitis(AP), and to investigate the changes of immune functional status in the development of acute pancreatitis. Methods An AP model was developed in adult male rats by retrograde injection of 4% sodium taurocholate in the pancreatic duct. T lymphocyte subsets( CD3 +, CD4 +, CD8 +) of peripheral blood and splenic cells were measured by Flow Cytometer. Results There was no significant change in the step of MAP. In the step of SAP, the level of CD3 + and CD4 + and CD4 +/CD8 + of peripheral blood was decreased, the decreasing of T lymphocyte subsets in splenic cells was more obvious than that in peripheral blood. Conclusion Spleen showed a negative immunologic function in the step of SAP and worsened SAP's development.