耳叶牛皮消对胃肠运动的作用及其机制研究

目的:研究耳叶牛皮消对胃肠道的药理作用及其机制。方法:用硫酸阿托品抑制小鼠小肠推进运动,观察耳叶牛皮消对小肠推进运动的影响;以夹尾刺激法制造大鼠功能性消化不良模型,观察耳叶牛皮消对模型大鼠胃黏膜一氧化氮(NO)含量的影响;用苦寒泻下法制造大鼠脾虚泄泻模型,观察耳叶牛皮消对模型大鼠血中胃肠激素水平的影响。结果:耳叶牛皮消能显著提高小肠运动受抑小鼠的小肠推进功能,显著降低功能性消化不良大鼠胃黏膜NO含量,显著升高脾虚泄泻模型大鼠血清胃泌素(GAS)和血浆胃动素(MTL)含量。结论:耳叶牛皮消促进胃肠运动的机制可能与其降低胃黏膜NO含量、提高血清GAS和血浆MTL水平有关。

Role and Mechanism of Cynanchum auriculatum on Gastrointestinal Motility

OBJECTIVE:To study the pharmacological action and mechanism of Cynanchum auriculatum(CYA)on the gastrointestinal tract.METHODS:Using atropine sulfate to inhibit mice's intestinal propulsive activity and the effect of CYA on the intestinal propulsive activity of mice was observed.Using clipping tail stimulation method to induce functional dyspepsia model in rats and the effect of CYA on nitrogen monoxidum(NO)level in gastric mucous membrane of the model rats was observed.Using cold and bitter rhubarb lax method to induce rat spleen-deficiency diarrhea model and the effect of CYA on levels of gastrin(GAS)and motilin(MTL)in the model rats was observed.RESULTS:CYA can apparently increase the intestinal propulsive activity of mice,markedly decrease the level of NO in gastric mucous membrane of functional dyspepsia model rats,and markedly increase levels of GAS in serum and MTL in plasma of spleen-deficiency diarrhea model rats.CONCLUSION:CYA can improve intestinal propulsive activity,which might be attributed to the decreased NO level in gastric mucous membrane and the elevated levels of GAS in serum and MTL in plasma.