EB病毒感染上调胃癌BGC823细胞的增殖和侵袭能力

目的 探讨EB病毒感染对胃癌细胞增殖状况和侵袭能力的影响。方法 用B95-8细胞体外培养获得EB病毒并感染人胃癌BGC823细胞，MTT法检测细胞增殖状况，羊膜侵袭实验检测细胞侵袭能力，流式细胞技术测定细胞周期变化，RT-PCR法检测感染后胃癌细胞中的病毒基因。结果 EB病毒感染胃癌BGC823细胞后，代表细胞增殖能力的4比值提高34．34％，体外侵袭羊膜的细胞数增加28．72％。S期细胞比例自36．7％增高为41．9％，G0／G1，期细胞比例减少，在EB病毒感染的BGC823细胞中可以检测到EB病毒核抗原1和EB病毒编码RNA1两种病毒基因，未检测到潜伏膜蛋白1和EB病毒核抗原2。结论 EB病毒感染能够促进胃癌细胞的增殖和侵袭能力、干扰其细胞周期，有可能在胃癌的发生、发展过程中发挥作用。

Epstein-Barr virus infection promotes the proliferation and invasiveness of a human gastric carcinoma cell line

Objective To explore the effect of EBV infection on proliferation and invasiveness of gastric cancer cell. Method BGC823, an EBV-negative human gastric cancer cell line, was infected by EBV. The cell proliferation and ability of invasion were studied by MTT method and in vitro invasive assay, respectively. Cell cycle was investigated bv flow cytometry. RT-PCR was used to detect the EBNAs, LMP1 and EBER1 in the infected cell. Result The A ratio of EBV-infected cells increased by 34. 34% and the population of invasive cells increased by 28.72%. The cell percentage of S phase, which was 36.7% at control group cell, was increased to 41.9% while the cell percentage of G0/G1 was decreased. EBNA1 and EBER1 were detected in EBV infected gastric cancer cells while EBNA2 and LMP1 were both negative. Conclusion Epstein-Barr virus infection promoted proliferation and upregulated invasion of human gastric cancer cell. EBV infection may play an important role in the carcinogenesis and progression of human gastric cancer.