定量分析乳腺癌术后化疗前外周血多药耐药基因及其临床意义的研究

目的探讨乳腺癌肿瘤组织多药耐药基因产物P170与外周血多药耐药基因MDR1mRNA的表达及乳腺癌组织ER、PR、癌基因、Her-2之间的相关性及其临床意义。方法应用荧光定量RT-PCR及免疫组化法对32例乳腺癌组织标本P170蛋白表达阳性的病人（试验组）和11例乳腺癌组织标本P170蛋白表达阴性的病人（对照组）进行化疗前的外周血液MDR1mRNA及ER、PR、Her-2检测。结果乳腺癌组织中多药耐药基因产物P170表达阳性的病人中，外周血MDR1mRNA表达阳性者14例（43．75％），阴性18例（56．25％）；乳腺癌组织中多药耐药基因产物P170表达阴性的11例病人中，外周血MDR1mRNA表达阳性者6例（54．55％），阴性5例（45．45％）；P170阳性组外周血MDR1mRNA阳性表达率43．75％与P170阴性组外周血MDR1mRNA阳性表达率54．55％之间差异无相关性（Χ^2=0．383，P〉0．05）。P170阳性组Her-2阳性表达12例（37．50％），阴性20例（62．50％）；P170阴性组Her-2阳性表达2例（18．18％），阴性9例（81．82％）；P170阳性组Her-2阳性表达率37．50％与P170阴性组Her-2阳性表达率18．18％之间差异无显著性（Χ^2=1．391，P〉0．05）。P170阳性组ER表达阳性者13例（40．63％），阴性19例（59．37％）；P170阴性组ER阳性表达者7例（63．64％），阴性4例（36．36％）；P170阳性组ER阳性表达率40．63％与P170阴性组ER阳性表达率63．64％之间差异无相关性（Χ^2=1．742，P〉0．05）。P170阳性组PR阳性表达者20例（62．50％），阴性12例（37．50％）；P170阴性组PR阳性表达者4例（36．36％），阴性7例（63．64％）；P170阳性组PR阳性表达率62．50％与P170阴性组PR阳性表达36．36％之间差异无相关性（Χ^2=2．267，P〉0．05）。P170阳性组淋巴结转移者9例（28．12％），无淋巴结转移23例（71．88％）；P170阴性组淋巴结转移者5例（45．45％），无淋巴结转移6?

Quantitative detection of peripheral blood MDR gene in breast cancer before chemotherapy and their clinical significances

Objective To study the expression of P170, MDR1 mRNA, Her-2, ER and PR in breast cancer, to evaluate their clinical significances, and to explore whether P170 and MDR1mRNA can become a kind of new index of diagnosis with breast cancer. Methods Fluorescent quantitation RT-PCR and immunohistochemistry was used to detect peripheral blood MDR1mRN, A before chemotherapy in breast cancer tissue samples from 32 cases with P170 positive （trial group） and 11 cases with P170 negative （control group）. Results There were 14 cases （43.75%） peripheral blood MDR1mRNA positive and 18 cases （56.25%） negative in 32 cases P170 positive breast cancer patients, while there were 6 cases （54.55%） peripheral blood MDRlmRNA positive and 5 cases（45.45%） negative in 11 cases P 170 negative breast cancer patients; There wasn＇t a significant difference with peripheral blood MDRlmRNA express rate in P170 positive（43.75%） group and P170 negative （54.55%） group （Χ^2=0.383, P〉0.05）. There were 12 cases（37.50%） Her-2 positive and 20 cases （62.50%） negative in P170 positive group; There were 2 cases（18.18%） Her-2 positive and 9 cases （81.82%） negative in P170 negative group; There wasn＇t a significant difference with Her-2 positive rate in P 170 positive group （37.50%） and P170 negative （18.18%） group （Χ^2=1.391, P〉0.05）. There were 13 cases （40.63%） ER positive and 19 cases （59.37%） negative in P 170 positive group; There were 7 cases （63.64%） ER positive and 4 cases （36.36%） negative in P170 negative group; There wasn＇t a significant difference with ER positive rate in P170 positive group （40.63%） and P170 negative （63.64%） group （Χ^2=1.742, P〉0.05）. There were 20 cases（62.50%） PR positive and 12 cases （37.50%） negative in P170 positive group; There were 4 cases（36.36%） PR positive and 7 cases （63.64%）negative in P 170 negative group; There wasn＇t a significance difference with PR positive rate in P170 positive group （62