SIGN-R1+MHC II+ cells of the splenic marginal zone--a novel type of resident dendritic cells |
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Authors: | Łyszkiewicz Marcin Zietara Natalia Rohde Manfred Gekara Nelson O Jabłońska Jadwiga Dittmar Kurt E Weiss Siegfried |
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Institution: | Hannover Medical School, Institute for Immunology, Carl-Neuberg-Str. 1 (OE5240), Hannover, Lower Saxony D-30625, Germany. marcin.lyszkiewicz@gmail.com |
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Abstract: | In the spleen, the MZ forms an interface between red and white pulp. Its major function is to trap blood-borne antigens and to reorient them to APCs and lymphocytes. SIGN-R1(+) cells are of the MZ inherent cell population, which for a long time, have been considered as macrophages. We now show that one subpopulation of SIGN-R1(+) cells that express MHC II molecules should be considered as a resident DC. Histological analysis indicated that SIGN-R1(+) cells have dendritic-like protrusions extending into T and B cell areas. Flow cytometry analysis revealed an expression profile of adhesion, costimulatory, and MHC molecules similar to cDCs but distinct from macrophages. Most importantly, SIGN-R1(+)MHC(+) cells were able to present antigen to na?ve CD4 T cells, as well as to cross-present soluble, particulate antigens secreted by Listeria monocytogenes to CD8 T cells in vitro and in vivo. Our experiments identified SIGN-R1(+)MHC II(+) cells as professional APCs and indicate their nature as splenic resident DCs. |
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