参白口服液对病毒性心肌炎心肌细胞凋亡及其相关调控基因影响的研究

目的：研究病毒性心肌炎（VMC）与心肌细胞凋亡及其相关调控基因的关系及参白口服液的防治作用。方法：给BALB/C小鼠接种嗜鼠心肌柯萨奇B3病毒（CVB3m）诱发VMC，在接种后3、7、14、20、30d动态对照观察心肌细胞凋亡（电镜、TUNEL）和相关调控基因C-myc、Bcl-2蛋白的表达（免疫组化技术）情况。结果：CVB3m可引起VMC心肌细胞凋亡，C-myc、Bcl-2蛋白的阳性表达支持心肌细胞凋亡情况。结论：心肌细胞凋亡及其调控基因可能参与VMC的发生和发展，参白口服液有良好的防治作用。

Study on Effect of Myocardial Cell Apoptosis and Its Regulatory Gene in Viral Myocarditis by Shenbai Oral Liquor

To suty the relationship of viral myocarditis (VMC) with myocardial cell apoptosis and its regulatory gene, and the effect of shenbai oral liquor on VMC. Method:The viral myocarditis in BALB/C mouse was obtained by coxsackie virus B 3m (CMB 3m ). The myocardial cell apoptosis and expressions of apoptosis regulatory gene c myc, Bcl 2 in various stages(the days of 3,7,14,20,30) of viral infection were detected by TDT/d UTP Nick End Labeling(TUNEL) and immunohistochemical method and electron microscope. Results:CVB 3m can Induse myocardial cell apoptosis of suckling Wistar rat. Increase in genetic expression of c myc and decrease in genetic expression of Bcl 2 in mouse myocardium support the finding of myocardial cell apoptosis. Conclusion:Myocardial cell apoptosis and its regulatory gene are partly related with development of VMC, and shenbai oral Liquor has significant functions of prevention and treatment.