| HPLC法测定感冒灵胶囊中咖啡因的含量 |
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| 引用本文： | 丁宏珍. HPLC法测定感冒灵胶囊中咖啡因的含量[J]. 安徽医药, 2009, 13(8): 887-888 |
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| 作者姓名： | 丁宏珍 |
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| 作者单位： | 安徽省淮南市食品药品检验所,安徽,淮南,232007 |
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| 摘 要： | 目的建立高效液相色谱法测定感冒灵胶囊中咖啡因含量的方法。方法采用Venusil MP-C18色谱柱;流动相：甲醇-水（25∶75）;流速：1.0 ml·min-1;检测波长：273 nm;柱温：20℃。结果咖啡因在0.07648-0.68832μg范围内呈良好的线性关系,r=1.0000,平均回收率99.6%,RSD为0.8%。结论该方法简便、准确、重现性好,可用于本品的质量控制。
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| 关 键 词： | HPLC 感冒灵胶囊 咖啡因 |
Determination of caffeine in ganmaoling capsule by HPLC |
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| DING Hong-zhen. Determination of caffeine in ganmaoling capsule by HPLC[J]. Anhui Medical and Pharmaceutical Journal, 2009, 13(8): 887-888 |
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| Authors: | DING Hong-zhen |
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| Affiliation: | DING Hong-zhen (Huainan Institute for Food , Drug Control,Huainan 232007,China) |
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| Abstract: | Aim To establish an HPLC method for the determination of caffeine in ganmaoling capsule.Methods The HPLC system consisted of Venusil MP-C18 column,methanol-water（25∶75） as a mobile phase,with a detection wavelength at 273 nm.The flow rate was 1.0 ml·min-1 and column temperature was 20℃.Results The linear range was between 0.07648 and 0.68832 μg,r=1.0000.The avery recoverage was 99.6% with RSD as 0.8%.Conclusion The method is simple,accurate and reliable,and can be used in the quality control of ganmaoling capsule. |
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| Keywords: | HPLC |
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