清热解毒中药对流感病毒FM1株感染所致小鼠肺组织病理损伤的影响

目的 动态观察清热解毒中药对流感病毒感染所致小鼠肺组织病理损伤的影响.方法 将96只健康雄性BALB/cAnN小鼠随机分为空白对照组、流感病毒感染模型组、中药治疗组,每组按照流感病毒感染1、3、5、7天不同时相再分为4个小组,每小组8只小鼠.用流感病毒亚洲甲型鼠肺适应株(FM1株)感染小鼠建立流感病毒性肺炎模型.在FM...

Effect of heat-clearing and detoxicating herbs on pathological injury of the influenza virus FM1 infected lung tissue of mice

Objective To observe dynamically the effect of heat-clearing and detoxicating herbs on the pathological injury of lung tissue in mice infected with influenza virus FM1.Methods Ninety-six healthy male BALB/cAnN mice were randomly divided into three group：normal control group,influenza infection model group and Chinese medicine treatment group.Each group was divided into 4 subgroups according to different time points（1,3,5 and 7 days after mice were infected with influenza virus）,8 mice in each subgroup.The model was made with the mice infected with FM1 influenza.During each phase of the infection,the mice in Chinese medicine treatment group were administered intragastrically with suspension of heat-clearing and detoxicating Herbs（Gypsum 30 g,Scutellaria 10 g,raw licorice 3 g,equivalent to adult clinical dose 5.58 g·kg-1·d-1）,meanwhile the mice in normal control group and influenza infection model group were administered intragastrically with the same amount of normal saline.On the 1st、3rd、5th and 7th day of infection,the mice were sacrificed and the lungs were take out.Then the dynamically pathological changes of lung tissue were observed with naked eyes,optical microscope and transmission electron microscopy respectively.Results According to the observation with naked eyes,the histological changes of lung in Chinese medicine treatment group were significantly slighter than those in influenza infection model group on day 3 and day 7,with a smaller amount of leakage,smaller range of focus and less lesions.According to the observation with optical microscope,the histological changes of lung in Chinese medicine treatment group were significantly alleviated in samller lung lesions,lower inflammatory cells density,less bronchioles epithelium loss,less inflammatory cells effusion in bronchial lumen and slighter endothelial cells of small blood vessels swelling.According to the observation with transmission electron microscopy,the histological changes of lung in Chinese medicine treatment group were significantly slighter than in influenza infection model group with alleviated destruction of pulmonary alveolar structure,proliferation of typeⅡ cells and basically normal form of Capillary endothelial cells in lung.Conclusion Heat-clearing and detoxicating herbs can improve the inflammatory disorder of lung in mice infected with FM1.It can also repair the pathological injury of lung tissue in mice infected with FM1.