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| SLP-76基因在消化道肿瘤中的表达差异分析 | |
| 引用本文： | 李鑫,陆佳韵,赵立群,王秀琴,刘桂林,刘忠,周传农,刘芝华,吴旻. SLP-76基因在消化道肿瘤中的表达差异分析[J]. 胃肠病学和肝病学杂志, 2003, 12(6): 551-552 |
| 作者姓名： | 李鑫 陆佳韵 赵立群 王秀琴 刘桂林 刘忠 周传农 刘芝华 吴旻 |
| 作者单位： | 1. 100011,北京,北京地坛医院感染科 2. 中国医学科学院肿瘤研究所分子肿瘤学国家重点实验室 3. 河南省医学科学研究所 4. 河南省辉县人民医院 |
| 基金项目： | 国家重点基础研究发展规划(G1 9980 51 0 2 1 )，国家自然科学基金(399934 0 2 )资助项目 |
| 摘 要： | 目的 分析SLP-76基因在食管癌、贲门癌、胃癌、结肠癌及其癌旁组织中的差异表达。方法 依据微阵列技术筛选出SLP-76基因在食管癌组织中高表达的结果，应用逆转录-聚合酶链式反应(RT-PCR)，对上述消化道肿瘤组织及其癌旁组织中的SLP-76基因表达进行检测。结果 SLP-76在73．3％(22／30)的食管癌组织中高表达(P=0．005)：在75％(9／12)的贲门癌组织中高表达(P=0．014)；在75％(12／16)胃癌组织中高表达(P=0．005)；在72．2％(13／18)结肠癌组织中高表达(P=0．008)。结论 在RNA水平，SLP-76基因在上述肿瘤之间的表达无显著性差异(P=0．9684)。但该基因在消化道肿瘤中高表达，SLP-76与上述消化道肿瘤生长关系密切；可能具有潜在的致癌性。 |
| 关 键 词： | SLP-76基因 消化道肿瘤 基因表达 微阵列技术 RT-PCR |
| 修稿时间： | 2003-03-10 |
Different expression and analysis of SLP-76 gene in cancer of the digestive tract and adjacent normal mucosa |
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| LI Xin,LU Jiayun,ZHAO Liqun,et alCancer Institute,Chinese Academy of Medical Sciences,Beijing ,China. Different expression and analysis of SLP-76 gene in cancer of the digestive tract and adjacent normal mucosa[J]. Chinese Journal of Gastroenterology and Hepatology, 2003, 12(6): 551-552 | |
| Authors: | LI Xin LU Jiayun ZHAO Liqun et alCancer Institute Chinese Academy of Medical Sciences Beijing China |
| Affiliation: | LI Xin,LU Jiayun,ZHAO Liqun,et alCancer Institute,Chinese Academy of Medical Sciences,Beijing 100021,China |
| Abstract: | Objective Confirm the microarray hybridization results and examine the expression of SLP-76 gene in digestive tract cancers and adjacent normal mucosa.MethodsAccording to analysis results of microarray, distinctly differential ly expressed SLP-76 gene was screening out. RT-PCR was applied to check the expression o f SLP-76 in digestive tract cancers and adjacent normal mucosa. ResultsBy RT-PCR examination ,we found that SLP-76 gene over expression in cancer of the digestive tract. ConclusionsDifferent expression of SLP-76 gene in cancer of the digestive tract is found, SLP-76 gene have closely relation with grown in the cancer of digestive tract.It is apossibly potential oncology gene in the cancer of digestive tract. This differential expressio n appeared no tissue specificity with statistical analysis (P=0.9684). |
| Keywords: | Digestive tract cancers SLP-76 gene Different expression |
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