浆细胞病患者IL-4产生细胞数减少的意义及机制探讨

本文运用APAAP法检测了16名浆细胞患者外周血细胞在PHA刺激下产生IL-4的能力。发现浆细胞病组阳性细胞比率明显低于正常对照组(P<0.001),且阳性细胞比率与患者血清中多克隆IgM、IgA水平呈正相关(r=0.806和r=0.703)。在正常人外周血中加入CRP50mg/L,经PHA刺激后IL-4产生细胞也明显减少。作者假设,由于IL-6增高引起CRP等急性期相蛋白大量分泌,使IL-4产生受到明显抑制,从而使B细胞激活发生障碍是浆细胞病中多克隆免疫球蛋白合成抑制的主要原因。

DECREASE IN IL-4 PRODUCING CELLS IN PLASMA CELL DYSCRASIAS: ITS SIGNIFICANCE AND POSSIBLE MECHANISM

In this paper, the capability of peripheral blood cells to produce IL-4 under the stimulation of PHA in 15 healthy controls and 16 patients with plasma cell dyscrasias was detected by APAAP method. It was found that positive cells were significantly less in patients than these in normal controls (P<0.001) (positively correlated to the serum polyclonal IgA and IgM concentrations). We also found that the patients' serum CRP concentrations were higher than those in normal controls (P<0.001). Adding 50mg/L CRP in the culture of normal peripheral blood made the IL-4 producing cells decrease greatly (P<0.001). The authors hypothesized that suppression of IL-4 production by increase in CRP and other acute phase proteins due to higher serum IL-6 activities in plasma cell dycrasias would result in defective activation of B cells, leading to decrease in synthesis of polyclonal immunoglobulins.