肾脏近曲小管上皮细胞囊泡膜H^＋—ATP酶的细胞化学定位

Cytochemical localization of H~+-ATPase on the vacuole membrane in epithelial cells of rat renal proximal tubules

This work was attempted to study cytochemically the localization of H+-ATPase on the vacuole membranein the epithelial cells of renal proximal tubules. In the incubation medium, P-NPP was used as the substrate, cerium asthe capture agent, and tricine-KOH as buffer to make a neutral condition. As a result, it is found that the enzyme activity Inside the matrix of lysosome can be inhibited by NaF, but insensitive to the enzyme activity on the lysosome and endosome membrane, the enzyme activity can be inhibited by NEM. The finding suggested that the enzyme activity on themembrane of lysosome and endosome was ouabain-insensitive and NEM-inhibitory proton pump H+-ATPase.