门冬氨酸钾镁对阿霉素中毒心肌细胞脂质过氧化钙离子过负荷的影响

应用阿霉素建立心肌细胞中毒模型，观察了门冬氨酸钾镁对中毒心肌保护作用并探讨了部分机制，结果显示：门冬氨酸钾镁能减少阿霉素中毒心肌细胞乳酸脱氢酶（ＬＤＨ的释放量，降低细胞内丙二醛（ＭＤＡ）含量及游离钙浓度，同时可升高细胞内超氧化物歧化酶（ＳＯＤ）活力，结果表明，门冬氨酸钾镁可能通过抗脂质氧化及逆转细胞内钙离子过负荷作用进而保护中毒心肌细胞。

The prevention of L-aspartate K-Mg on lipid peroxidation and Calcium overloading in toxic cardial cell induced by doxorubicin

The purpose of this study was to expore the prevention of doxorubicin-induced toxic cardiral cell with L- aspartate K-Mg. The activity of superoxide dismutase(SOD) in the myocardial cells was determined by the diphenol method, plasma malondialdehyde(MDA) content was measured by TBA assay, the culture medium LDH content was measured by the acetonic acid method, the intracellular calcium concentration was measured by fluorescence spectropho- tometer- The results was shown that aspartate-K. Mg increased the activity of SOD, decreased the content of MDA in plasma and the activity of LOH in the culture medium in toxic myocardial cell induced by doxorubicin. In additions, L- aspartate K-Mg was shown to reduce the intracellular Ca2+ overloading induced by doxorubicin. It revealed that L-as- partate K-Mg could keep myocardial cells from damage induced by doxorubicin.